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简化的电子显微镜网格玻璃化是一种用于小鼠成熟卵母细胞冷冻保存的便捷高效方法。

Simplified EM grid vitrification is a convenient and efficient method for mouse mature oocyte cryopreservation.

作者信息

Kim Seok Hyun, Ku Seung-Yup, Sung Ki Cheong, Kang Moon Joo, Kim Sung Ah, Kim Hee Sun, Oh Sun Kyung, Jee Byung Chul, Suh Chang Suk, Choi Young Min, Kim Jung Gu, Moon Shin Yong

机构信息

Department of Obstetrics and Gynecology, Seoul National University, Chongno-gu, Seoul 110-744, Korea.

出版信息

Yonsei Med J. 2006 Jun 30;47(3):399-404. doi: 10.3349/ymj.2006.47.3.399.

DOI:10.3349/ymj.2006.47.3.399
PMID:16807991
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2688161/
Abstract

This study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compared with those of the slow freezing method using 1.5M DMSO. Then, conventional EM grid vitrification was compared with simplified EM grid vitrification. Simplified EM grid vitrification showed higher survival, fertilization and hatching rates than those of the slow freezing method (85.6% vs. 63.2%; 51.0% vs. 22.3%; 38.7% vs. 12.5%, p < 0.01, respectively). Moreover, simplified EM grid vitrification showed higher recovery, survival and fertilization rates than those of conventional EM grid vitrification (100% vs. 95.0%, p=0.024; 90.0% vs. 78.9%, p=0.033; 56.7% vs. 38.7%, p=0.021, respectively). Hatching rate tended to be higher for simplified EM grid vitrification compared to conventional EM grid vitrification (41.1% vs. 24.1%). In conclusion, simplified EM grid vitrification is a convenient and efficient method for cryopreservation of mouse mature oocytes, compared to conventional EM grid vitrification and slow freezing methods.

摘要

本研究旨在评估简化的电子显微镜网格玻璃化法的效率，该方法在加载卵母细胞后跳过了去除网格上冷冻保护剂（5.5M乙二醇+1.0M蔗糖）液滴的步骤，并与小鼠成熟卵母细胞的传统冷冻保存方案进行比较。首先，将简化的电子显微镜网格玻璃化法的复苏、存活、受精和孵化率与使用1.5M二甲基亚砜的慢速冷冻法进行比较。然后，将传统的电子显微镜网格玻璃化法与简化的电子显微镜网格玻璃化法进行比较。简化的电子显微镜网格玻璃化法的存活率、受精率和孵化率均高于慢速冷冻法（分别为85.6%对63.2%；51.0%对22.3%；38.7%对12.5%，p<0.01）。此外，简化的电子显微镜网格玻璃化法的复苏、存活和受精率高于传统的电子显微镜网格玻璃化法（分别为100%对95.0%，p=0.024；90.0%对78.9%，p=0.033；56.7%对38.7%，p=0.021）。与传统的电子显微镜网格玻璃化法相比，简化的电子显微镜网格玻璃化法的孵化率有升高趋势（41.1%对24.1%）。总之，与传统的电子显微镜网格玻璃化法和慢速冷冻法相比，简化的电子显微镜网格玻璃化法是一种方便、高效的小鼠成熟卵母细胞冷冻保存方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65d3/2688161/e4456c7ce4a9/ymj-47-399-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65d3/2688161/e4456c7ce4a9/ymj-47-399-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65d3/2688161/e4456c7ce4a9/ymj-47-399-g001.jpg

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Nanomedicine (Lond). 2011 Aug;6(6):1115-29. doi: 10.2217/nnm.11.71.
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