Martins Vanessa, Asad Yasmin, Wilsher Nicola, Raynaud Florence
Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Nov 7;843(2):262-7. doi: 10.1016/j.jchromb.2006.06.010. Epub 2006 Jun 30.
A sensitive and selective LC-MS/MS method has been developed and validated for the quantification of abiraterone acetate and its metabolite, abiraterone (an androgen biosynthesis inhibitor) in human plasma. Analytes were extracted by SPE with cation mixed-mode polymer cartridges. Chromatography was performed on a Luna C5 5 microm, 50 mm x 2.1 mm i.d. column, using a mobile phase of 2% propan-2-ol in acetonitrile and 10mM ammonium acetate. The assay was linear from 5 to 500 nM (r(2)=0.998). The intra- and inter-day coefficients of variation were <13.9% for both analytes. This method will be applied to a clinical trial investigating the pharmacokinetics of abiraterone acetate and abiraterone in patients with prostate cancer.
已开发并验证了一种灵敏且具选择性的液相色谱-串联质谱法，用于定量测定人血浆中醋酸阿比特龙及其代谢物阿比特龙（一种雄激素生物合成抑制剂）。分析物采用阳离子混合模式聚合物柱通过固相萃取法进行提取。色谱分析在Luna C5 5微米、50毫米×2.1毫米内径的色谱柱上进行，流动相为乙腈中含2%的异丙醇和10毫摩尔醋酸铵。该测定法在5至500纳摩尔范围内呈线性（r(2)=0.998）。两种分析物的日内和日间变异系数均<13.9%。该方法将应用于一项关于前列腺癌患者中醋酸阿比特龙和阿比特龙药代动力学的临床试验。
