Ren Dongfeng, Hou Shuxun, Wang Huadong, Luo Dianzhong, Zhang Lihai
Department of Orthopaedics, Institute of Orthopaedics, Beijing, PR China.
Artif Cells Blood Substit Immobil Biotechnol. 2006;34(3):293-303. doi: 10.1080/10731190600683829.
To observe the changes during the process of modificating collagen with Arg-Gly-Asp (RGD) peptide, a series experiments were designed.
The 3D porous collagen matrices modified with RGD peptide were constructed by lyophilization. The modified and unmodified matrices were characterized by Scanning Electron Microscopy (SEM) and Electron Spectroscopy for Chemical Analysis (ESCA). Fibroblasts were used to evaluate the cell compatibility of the matrices.
In terms of cell growth, the cells attached much better on the modified matrix than on the unmodified one. Compare to the unmodified matrices, the polar groups on the modified matrix increased.
The introducing of specific RGD receptor-mediated adhesion site on matrices obviously enhanced the cells adhesion on collagen matrices.
为观察用精氨酸-甘氨酸-天冬氨酸（RGD）肽修饰胶原蛋白过程中的变化，设计了一系列实验。
通过冻干构建用RGD肽修饰的三维多孔胶原蛋白基质。用扫描电子显微镜（SEM）和化学分析电子能谱（ESCA）对修饰和未修饰的基质进行表征。用成纤维细胞评估基质的细胞相容性。
在细胞生长方面，细胞在修饰基质上的附着比在未修饰基质上要好得多。与未修饰基质相比，修饰基质上的极性基团增加。
在基质上引入特定的RGD受体介导的粘附位点明显增强了细胞在胶原蛋白基质上的粘附。
