Caroline Bruschi Alonso Roberta, Maria Correr Gisele, Gonçalves Cunha Leonardo, Flávia Sanches Borges Ana, Maria Puppin-Rontani Regina, Alexandre Coelho Sinhoreti Mário
Dental Materials Area, Department of Restorative Dentistry, Piracicaba Dental School, UNICAMP, Piracicaba, SP, Brazil.
Acta Odontol Scand. 2006 Jun;64(3):141-5. doi: 10.1080/00016350500474565.
The aim of this study was to evaluate and compare marginal adaptation of composite restorations assessed by a dye staining method and by scanning electron microscopy (SEM) analysis.
Twenty bovine incisors were selected and ground flat to expose dentin. Two cylindrical cavities were prepared on the central area of flattened surfaces. Single bond adhesive system was applied in accordance with the manufacturer's instructions and the cavities were filled with Filtek Z250 or Filtek Flow. The specimens were polished and replicas were obtained in epoxy resin. The replicas were observed by SEM for marginal quality/quantity evaluation. Caries detector was then applied on each specimen for 5 s to verify marginal adaptation through dye staining of the formed gaps on the outer margins. Images of the stained gaps were transferred to a computer measurement program to determine gap length. The length of the gap was expressed as the percentage of total length of the margins observed. Data were submitted to two-way ANOVA and Pearson correlation.
Filtek flow showed 36% and 34% and Filtek Z250 27% and 29% of gap in the margins when evaluated by SEM analysis and by the dye staining test, respectively. There was no difference between the composites, regardless of the evaluation technique. There was a strong positive correlation (r=0.83) between the results obtained with the tested methods to assess marginal gap.
Dye staining the gaps can be used with good reliability to evaluate the gap formation in composite restorations.
本研究旨在评估和比较通过染料染色法和扫描电子显微镜（SEM）分析评估的复合树脂修复体的边缘适应性。
选取20颗牛切牙并磨平以暴露牙本质。在磨平表面的中央区域制备两个圆柱形洞腔。按照制造商的说明应用单组分粘结剂系统，并用Filtek Z250或Filtek Flow填充洞腔。对标本进行抛光，并在环氧树脂中制作复制品。通过扫描电子显微镜观察复制品以评估边缘质量/数量。然后在每个标本上应用龋病检测剂5秒，通过对边缘形成的间隙进行染料染色来验证边缘适应性。将染色间隙的图像传输到计算机测量程序以确定间隙长度。间隙长度表示为观察到的边缘总长度的百分比。数据进行双向方差分析和Pearson相关性分析。
通过扫描电子显微镜分析和染料染色试验评估时，Filtek Flow在边缘处的间隙分别为36%和34%，Filtek Z250为27%和29%。无论评估技术如何，复合材料之间均无差异。在评估边缘间隙的测试方法所获得的结果之间存在强正相关（r = 0.83）。
对间隙进行染料染色可可靠地用于评估复合树脂修复体中的间隙形成。
