细胞核重编程与多能性

Nuclear reprogramming and pluripotency.

作者信息

Hochedlinger Konrad, Jaenisch Rudolf

机构信息

Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

出版信息

Nature. 2006 Jun 29;441(7097):1061-7. doi: 10.1038/nature04955.

DOI:10.1038/nature04955

PMID:16810240

Abstract

The cloning of mammals from differentiated donor cells has refuted the old dogma that development is an irreversible process. It has demonstrated that the oocyte can reprogramme an adult nucleus into an embryonic state that can direct development of a new organism. The prospect of deriving patient-specific embryonic stem cells by nuclear transfer underscores the potential use of this technology in regenerative medicine. The future challenge will be to study alternatives to nuclear transfer in order to recapitulate reprogramming in a Petri dish without the use of oocytes.

摘要

从分化的供体细胞克隆哺乳动物，驳斥了发育是一个不可逆过程的旧教条。它表明，卵母细胞可以将成年细胞核重编程为胚胎状态，从而指导新生物体的发育。通过核移植获得患者特异性胚胎干细胞的前景，凸显了这项技术在再生医学中的潜在用途。未来的挑战将是研究核移植的替代方法，以便在不使用卵母细胞的情况下在培养皿中重现重编程过程。