Basto Renata, Lau Joyce, Vinogradova Tatiana, Gardiol Alejandra, Woods C Geoffrey, Khodjakov Alexey, Raff Jordan W
The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, UK.
Cell. 2006 Jun 30;125(7):1375-86. doi: 10.1016/j.cell.2006.05.025.
Centrioles and centrosomes have an important role in animal cell organization, but it is uncertain to what extent they are essential for animal development. The Drosophila protein DSas-4 is related to the human microcephaly protein CenpJ and the C. elegans centriolar protein Sas-4. We show that DSas-4 is essential for centriole replication in flies. DSas-4 mutants start to lose centrioles during embryonic development, and, by third-instar larval stages, no centrioles or centrosomes are detectable. Mitotic spindle assembly is slow in mutant cells, and approximately 30% of the asymmetric divisions of larval neuroblasts are abnormal. Nevertheless, mutant flies develop with near normal timing into morphologically normal adults. These flies, however, have no cilia or flagella and die shortly after birth because their sensory neurons lack cilia. Thus, centrioles are essential for the formation of centrosomes, cilia, and flagella, but, remarkably, they are not essential for most aspects of Drosophila development.
中心粒和中心体在动物细胞组织中起着重要作用，但它们对动物发育的重要程度尚不确定。果蝇蛋白DSas-4与人类小头畸形蛋白CenpJ及秀丽隐杆线虫的中心粒蛋白Sas-4相关。我们发现DSas-4对果蝇的中心粒复制至关重要。DSas-4突变体在胚胎发育过程中开始丢失中心粒，到三龄幼虫阶段时，已检测不到任何中心粒或中心体。突变细胞中的有丝分裂纺锤体组装缓慢，幼虫神经母细胞约30%的不对称分裂异常。然而，突变果蝇仍能在接近正常的时间发育成形态正常的成虫。不过，这些果蝇没有纤毛或鞭毛，出生后不久便死亡，因为它们的感觉神经元缺乏纤毛。因此，中心粒对于中心体、纤毛和鞭毛的形成至关重要，但值得注意的是，它们对果蝇发育的大多数方面并非必不可少。
