Ristanović Dusan, Milosević Nebojsa T, Stulić Vesna
Department of Biophysics, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia and Montenegro.
J Neurosci Methods. 2006 Dec 15;158(2):212-8. doi: 10.1016/j.jneumeth.2006.05.030. Epub 2006 Jun 30.
The drawings of Golgi-impregnated neurons from laminae I to VI in dorsal horn of the cat spinal cord were analysed morphometrically with a modified Sholl method of concentric circles. In order to advance the Sholl analysis of neuronal dendritic arborization patterns, we developed a new method of data presentation using polynomial regression and defining three parameters: the critical value of the circle radius (which defines the place of a possible circle intersecting maximum number of dendrites), the maximum number of dendritic intersections with the circles (counted for consecutive circles placed starting at the cell body to the border of the dendritic tree), and the mean value of the fitted polynomial function (which describes an average property concerning numbers of branches of dendritic tree over the whole region occupied by the dendritic arbor). For that purpose we also used the Sholl regression coefficient as well as the Schoenen ramification index. As an illustration of our model, we demonstrate that proposed modification of the Sholl method can successfully discriminate neuronal populations among different laminae of the cat spinal cord.
采用改良的同心圆式肖尔(Sholl)方法,对猫脊髓背角Ⅰ至Ⅵ层经高尔基(Golgi)浸染的神经元绘图进行形态测量分析。为推进对神经元树突分支模式的肖尔分析,我们开发了一种新的数据呈现方法,该方法使用多项式回归并定义三个参数:圆半径的临界值(定义可能与最大数量树突相交的圆的位置)、与圆的树突交叉的最大数量(从细胞体开始到树突树边界放置的连续圆计算)以及拟合多项式函数的平均值(描述树突分支在树突分支占据的整个区域内的平均特性)。为此,我们还使用了肖尔回归系数以及舍嫩(Schoenen)分支指数。作为我们模型的一个例证,我们证明所提出的肖尔方法修改能够成功区分猫脊髓不同层中的神经元群体。