[2004 - 2005年在中国流行的B型流感病毒的抗原性和基因研究]

[Antigenic and genetic study of influenza virus B circulated in China in 2004-2005].

作者信息

Zhang Ye, Wen Le-ying, Zhao Xiang, Li Zi, Guo Jun-feng, Xu Cui-ling, Wang Min, Yu Hong-jie, Yang Wei-zhong, Guo Yuan-ji, Shu Yue-long

机构信息

Chinese Influenza Center, Institute for Viral Disease Control and Prevention, State Key Laboratory for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2006 Jun;20(2):11-3.

PMID:16816852

Abstract

BACKGROUND

To analyze the genetic and antigenic characteristics of hemagglutinin (HA) gene of human influenza B virus isolated from the mainland of China since 2004-2005.

METHODS

The single-way hemagglutinin inhibition (HI) tests were used to test the antigenic characteristics, and the HA1 gene was sequenced based on the antigenic results.

RESULTS

The Yamagata-like and Victoria-like viruses co-circulated in 2004-2005. For the Yamagata-like virus, the single-way HI results showed that 3.7% and 4.5% of the viruses had 4-fold greater HI titer difference compared with B/Shanghai/361/02 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had amino acid mutation, and there was one more glycosylation site at 196th site. For the Victoria-like virus, the single-way HI results showed that 8.5% and 20.6% of the viruses had 4-fold greater HI titer difference compared with B/Hong kong/330/01 in 2004 and 2005, respectively. The HA1 sequence data showed that the virus had replacement of 9 amino acids, and there was one more glycosylation site at 197th site.

CONCLUSION

The results showed that influenza B viruses had changed antigenic and genetic characteristics compared with B/Shanghai/361/02, B/Hong kong/330/01 in 2004-2005.

摘要

背景

分析2004 - 2005年以来从中国大陆分离的乙型流感病毒血凝素（HA）基因的遗传和抗原特性。

方法

采用单向血凝抑制（HI）试验检测抗原特性，并根据抗原结果对HA1基因进行测序。

结果

2004 - 2005年山形株和维多利亚株病毒共同流行。对于山形株病毒，单向HI结果显示，2004年和2005年分别有3.7%和4.5%的病毒与B/上海/361/02相比HI效价差异达4倍以上。HA1序列数据显示该病毒存在氨基酸突变，且在第196位多了一个糖基化位点。对于维多利亚株病毒，单向HI结果显示，2004年和2005年分别有8.5%和20.6%的病毒与B/香港/330/01相比HI效价差异达4倍以上。HA1序列数据显示该病毒有9个氨基酸替换，且在第197位多了一个糖基化位点。