State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease; Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, 151 Yan Jiang Road, Guangzhou, Guangdong, 510120, People's Republic of China.
Virol J. 2018 Nov 14;15(1):175. doi: 10.1186/s12985-018-1085-5.
Few studies have analyzed influenza B virus lineages based on hemagglutinin A (HA) gene sequences in southern China. The present study analyzed the HA gene and the lineages of influenza B virus isolates from Guangzhou during 2016, and compared our results with the WHO-recommended vaccine strain.
Ninety patients with influenza B were recruited from the First Hospital of Guangzhou Medical University. Throat swab specimens of 72 patients had high viral loads. Among these 72 isolates, the HA1 domain of the HA gene in 43 randomly selected isolates was sequenced using reverse transcription-polymerase chain reaction (RT-PCR), and analyzed using MEGA 5.05.
Eight of the 90 patients (8.9%) also had influenza A virus infections. Analysis of the 43 influenza B virus isolates indicated that 34 (79.1%) were from the Victoria lineage and 9 (20.9%) were from the Yamagata lineage. A comparison isolates in our Victoria lineage with the B/Brisbane/60/2008 strain indicated 12 mutation sites in the HA1 domain, 4 of which (I132V, N144D, C196S, and E198D) were in antigenic epitopes. A comparison of isolates in our Yamagata lineage with the B/Phuket/3073/2013 stain indicated 5 mutation sites in the HA1 domain, none of which was in an antigenic epitope. None of the isolates had a mutation in regions of the neuraminidase gene (NA) that are known to confer resistance to NA inhibitors.
In Guangzhou during 2016, most influenza B virus isolates were from the Victoria lineage, in contrast to the vaccine strain recommended by the WHO for this period.
在中国南方，很少有研究基于血凝素 A（HA）基因序列分析乙型流感病毒谱系。本研究分析了广州 2016 年乙型流感病毒分离株的 HA 基因和谱系，并将我们的结果与世界卫生组织推荐的疫苗株进行了比较。
从广州医科大学第一附属医院招募了 90 名乙型流感患者。72 名患者的咽拭子标本病毒载量较高。在这 72 个分离物中，随机选择的 43 个分离物的 HA 基因的 HA1 结构域使用逆转录-聚合酶链反应（RT-PCR）进行测序，并使用 MEGA 5.05 进行分析。
90 名患者中有 8 名（8.9%）还感染了甲型流感病毒。对 43 株乙型流感病毒分离株的分析表明，34 株（79.1%）来自维多利亚谱系，9 株（20.9%）来自 Yamagata 谱系。我们的维多利亚谱系中的分离株与 B/Brisbane/60/2008 株的比较表明，HA1 结构域中有 12 个突变位点，其中 4 个（I132V、N144D、C196S 和 E198D）位于抗原表位中。我们的 Yamagata 谱系中的分离株与 B/Phuket/3073/2013 株的比较表明，HA1 结构域中有 5 个突变位点，均不在抗原表位中。神经氨酸酶基因（NA）的 NA 抑制剂耐药区域没有分离株发生突变。
2016 年在广州，大多数乙型流感病毒分离株来自维多利亚谱系，与世界卫生组织推荐的该时期疫苗株不同。
