Adah Ameze, Benghuzzi Hamed, Tucci Michelle, Huang Derek, Franklin Laura, Adah Felix
University of Mississippi, University, MS 38677, USA.
Biomed Sci Instrum. 2006;42:120-5.
Glycolysis is a very important process which contains very intricate steps that play a role in cellular performance and viability. Fructose 1,6-bisphosphate (FBP) is a glycolytic intermediate that has proven to improve cellular conditions under hypoxic and ischemic conditions. Osteoblasts are key regulators of skeletal matrix synthesis and degradation. Thus, considering FBP's positive effects on ameliorating hypoxia-induced injuries, the objective of this study was to determine its effects and comparative effects on osteoblast cells under normoxic and hypoxic states. MG63 osteoblast-like cells were cultured in 24-well culture plates and treated with high, medium and low dosages of FBP at 24, 48, and 72 hours. At the end of each time period, cellular number, damage by a malondialdehyde assay (MDA), and glutathione levels were evaluated. There was a significant increase in cell number for the low level of FBP in normoxia at 48 hours (p < 0.05). For the cells in hypoxia, there was a significant decrease in cell number for the medium level at 48 hours (p < 0.05). At 48 hours there was a significant decrease in cell damage through MDA measurement for the cells in normoxia and hypoxia when compared to the control. Cellular damage was not evident in the supernatant in either oxygen condition for the duration of the study. A significant decrease in glutathione levels was also noted for the cells in hypoxia. Cellular morphology included multiple nucleoli, vacuolated cytoplasm, abnormal cells, and web-like cytoplasm. The results indicate that FBP does protect bone cells exposed to hypoxic injuries, and while doing so, ameliorating the states of the cells in shock.
糖酵解是一个非常重要的过程，包含非常复杂的步骤，这些步骤在细胞性能和活力方面发挥作用。1,6-二磷酸果糖（FBP）是一种糖酵解中间产物，已被证明在缺氧和缺血条件下可改善细胞状况。成骨细胞是骨骼基质合成和降解的关键调节因子。因此，考虑到FBP对改善缺氧诱导损伤的积极作用，本研究的目的是确定其在常氧和低氧状态下对成骨细胞的影响及比较效果。将MG63成骨样细胞培养在24孔培养板中，并在24、48和72小时用高、中、低剂量的FBP处理。在每个时间段结束时，评估细胞数量、丙二醛测定（MDA）造成的损伤以及谷胱甘肽水平。在常氧状态下，48小时时低剂量FBP处理组的细胞数量显著增加（p < 0.05）。对于缺氧细胞，48小时时中剂量组的细胞数量显著减少（p < 0.05）。与对照组相比，48小时时，通过MDA测量发现常氧和缺氧条件下的细胞损伤均显著降低。在整个研究期间，两种氧条件下的上清液中均未观察到明显的细胞损伤。缺氧细胞的谷胱甘肽水平也显著降低。细胞形态包括多个核仁、空泡化细胞质、异常细胞和网状细胞质。结果表明，FBP确实能保护暴露于缺氧损伤的骨细胞，同时改善休克状态下的细胞状态。
