延髓头端腹内侧区脊髓投射神经元中的谷氨酸

Glutamate in spinally projecting neurons of the rostral ventral medulla.

作者信息

Minson J, Pilowsky P, Llewellyn-Smith I, Kaneko T, Kapoor V, Chalmers J

机构信息

Department of Medicine and Centre for Neuroscience, Flinders University of South Australia, Adelaide.

出版信息

Brain Res. 1991 Aug 2;555(2):326-31. doi: 10.1016/0006-8993(91)90359-4.

DOI:10.1016/0006-8993(91)90359-4

PMID:1682002

Abstract

Phosphate activated glutaminase (PAG), an enzyme of glutamate synthesis, was localized by immunohistochemistry in all PNMT-immunoreactive and all serotonin-immunoreactive neurons in the rostral ventral medulla of the rat. Between 71 and 83% of bulbospinal neurons localised in the rostral ventral medulla projecting to the intermediolateral cell column in the upper thoracic spinal cord contained PAG immunoreactivity. Of these bulbospinal PAG-immunoreactive neurons 17-27% contained PNMT immunoreactivity and 9-16% contained serotonin immunoreactivity. Other bulbospinal PAG-immunoreactive neurons (60-70%) contained neither PNMT- nor serotonin immunoreactivity. The results provide anatomical evidence suggestive of a glutamatergic input to the sympathetic preganglionic neurons of the spinal cord arising from different populations of neurons located in the rostral ventral medulla.

摘要

磷酸激活谷氨酰胺酶（PAG）是一种谷氨酸合成酶，通过免疫组织化学法在大鼠延髓腹侧吻部所有含苯乙醇胺N-甲基转移酶（PNMT）免疫反应性的神经元和所有含5-羟色胺免疫反应性的神经元中定位。位于延髓腹侧吻部并投射到上胸段脊髓中间外侧细胞柱的延髓脊髓神经元中，71%至83%含有PAG免疫反应性。在这些含PAG免疫反应性的延髓脊髓神经元中，17% - 27%含有PNMT免疫反应性，9% - 16%含有5-羟色胺免疫反应性。其他含PAG免疫反应性的延髓脊髓神经元（60% - 70%）既不含有PNMT免疫反应性也不含有5-羟色胺免疫反应性。这些结果提供了解剖学证据，提示脊髓交感神经节前神经元存在来自位于延髓腹侧吻部不同神经元群体的谷氨酸能输入。