Amharref Nadia, Beljebbar Abdelilah, Dukic Sylvain, Venteo Lydie, Schneider Laurence, Pluot Michel, Vistelle Richard, Manfait Michel
Unité MéDIAN, CNRS-UMR 6142, UFR de Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims cedex, France.
Biochim Biophys Acta. 2006 Jul;1758(7):892-9. doi: 10.1016/j.bbamem.2006.05.003. Epub 2006 May 16.
Pathological changes associated with the development of brain tumor were investigated by Fourier transform infrared microspectroscopy (FT-IRM) with high spatial resolution. Using multivariate statistical analysis and imaging, all normal brain structures were discriminated from tumor and surrounding tumor tissues. These structural changes were mainly related to qualitative and quantitative changes in lipids (tumors contain little fat) and were correlated to the degree of myelination, an important factor in several neurodegenerative disorders. Lipid concentration and composition may thus be used as spectroscopic markers to discriminate between healthy and tumor tissues. Additionally, we have identified one peculiar structure all around the tumor. This structure could be attributed to infiltrative events, such as peritumoral oedema observed during tumor development. Our results highlight the ability of FT-IRM to identify the molecular origin that gave rise to the specific changes between healthy and diseased states. Comparison between pseudo-FT-IRM maps and histological examinations (Luxol fast blue, Luxol fast blue-cresyl violet staining) showed the complementarities of both techniques for early detection of tissue abnormalities.
利用具有高空间分辨率的傅里叶变换红外光谱显微镜（FT-IRM）研究了与脑肿瘤发生相关的病理变化。通过多元统计分析和成像，所有正常脑结构均与肿瘤及肿瘤周围组织区分开来。这些结构变化主要与脂质的定性和定量变化有关（肿瘤含少量脂肪），并与髓鞘形成程度相关，髓鞘形成程度是几种神经退行性疾病中的一个重要因素。因此，脂质浓度和组成可用作光谱标记物，以区分健康组织和肿瘤组织。此外，我们在肿瘤周围发现了一种特殊结构。这种结构可能归因于浸润性事件，如肿瘤发展过程中观察到的瘤周水肿。我们的结果突出了FT-IRM识别导致健康状态和疾病状态之间特定变化的分子起源的能力。伪FT-IRM图谱与组织学检查（卢戈氏快蓝、卢戈氏快蓝-甲酚紫染色）之间的比较显示了两种技术在早期检测组织异常方面的互补性。
