腺嘌呤与大肠杆菌O157:H7的蛋白质毒素Stx2的结合。
Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7.
作者信息
Fraser Marie E, Cherney Maia M, Marcato Paola, Mulvey George L, Armstrong Glen D, James Michael N G
机构信息
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary AB T2N 1N4, Canada.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt 7):627-30. doi: 10.1107/S1744309106021968. Epub 2006 Jun 26.
Abstract
Stx2 is a protein toxin whose catalytic subunit acts as an N-glycosidase to depurinate a specific adenine base from 28S rRNA. In the holotoxin, the catalytic portion, A1, is linked to the rest of the A subunit, A2, and A2 interacts with the pentameric ring formed by the five B subunits. In order to test whether the holotoxin is active as an N-glycosidase, Stx2 was crystallized in the presence of adenosine and adenine. The crystals diffracted to approximately 1.8 angstroms and showed clear electron density for adenine in the active site. Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA.
摘要
志贺毒素2（Stx2）是一种蛋白质毒素，其催化亚基作为N-糖苷酶，从28S核糖体RNA上去除特定的腺嘌呤碱基。在全毒素中，催化部分A1与A亚基的其余部分A2相连，并且A2与由五个B亚基形成的五聚体环相互作用。为了测试全毒素作为N-糖苷酶是否具有活性，在腺苷和腺嘌呤存在的情况下使Stx2结晶。晶体衍射至约1.8埃，并在活性位点显示出腺嘌呤的清晰电子密度。腺苷已被切割，证明Stx2是一种活性N-糖苷酶。虽然全毒素对小分子底物具有活性，但可以预期B亚基会干扰28S核糖体RNA的结合。
相似文献
1
Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt 7):627-30. doi: 10.1107/S1744309106021968. Epub 2006 Jun 26.
2
Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7.
J Biol Chem. 2004 Jun 25;279(26):27511-7. doi: 10.1074/jbc.M401939200. Epub 2004 Apr 9.
3
Do the A subunits contribute to the differences in the toxicity of Shiga toxin 1 and Shiga toxin 2?
Toxins (Basel). 2015 Apr 29;7(5):1467-85. doi: 10.3390/toxins7051467.
4
Top-down proteomic identification of furin-cleaved α-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS.
J Biomed Biotechnol. 2010;2010:123460. doi: 10.1155/2010/123460. Epub 2011 Feb 10.
5
The serine 31 residue of the B subunit of Shiga toxin 2 is essential for secretion in enterohemorrhagic Escherichia coli.
Infect Immun. 2007 May;75(5):2189-200. doi: 10.1128/IAI.01546-06. Epub 2007 Feb 26.
6
Regulatory elements of stx2 gene and the expression level of Shiga-like toxin 2 in Escherichia coli O157:H7.
J Microbiol Immunol Infect. 2018 Feb;51(1):132-140. doi: 10.1016/j.jmii.2016.04.006. Epub 2016 May 13.
7
Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection.
Infect Immun. 2002 Feb;70(2):612-9. doi: 10.1128/IAI.70.2.612-619.2002.
8
Differences in Ribosome Binding and Sarcin/Ricin Loop Depurination by Shiga and Ricin Holotoxins.
Toxins (Basel). 2017 Apr 11;9(4):133. doi: 10.3390/toxins9040133.
9
Cinnamon Oil Inhibits Shiga Toxin Type 2 Phage Induction and Shiga Toxin Type 2 Production in Escherichia coli O157:H7.
Appl Environ Microbiol. 2016 Oct 27;82(22):6531-6540. doi: 10.1128/AEM.01702-16. Print 2016 Nov 15.
10
Development and characterization of recombinant antibody fragments that recognize and neutralize in vitro Stx2 toxin from Shiga toxin-producing Escherichia coli.
PLoS One. 2015 Mar 19;10(3):e0120481. doi: 10.1371/journal.pone.0120481. eCollection 2015.
引用本文的文献
1
Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli.
Sci Rep. 2025 May 14;15(1):16712. doi: 10.1038/s41598-025-01342-2.
2
Synthesis of biologically active Shiga toxins in cell-free systems.
Sci Rep. 2024 Mar 13;14(1):6043. doi: 10.1038/s41598-024-56190-3.
3
A unique peptide-based pharmacophore identifies an inhibitory compound against the A-subunit of Shiga toxin.
Sci Rep. 2022 Jul 6;12(1):11443. doi: 10.1038/s41598-022-15316-1.
4
Identification of a peptide motif that potently inhibits two functionally distinct subunits of Shiga toxin.
Commun Biol. 2021 May 10;4(1):538. doi: 10.1038/s42003-021-02068-3.
5
Structural basis for the interaction of Shiga toxin 2a with a C-terminal peptide of ribosomal P stalk proteins.
J Biol Chem. 2020 Nov 13;295(46):15588-15596. doi: 10.1074/jbc.AC120.015070. Epub 2020 Sep 2.
6
Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome.
Toxins (Basel). 2020 Jun 4;12(6):373. doi: 10.3390/toxins12060373.
7
Differences in Ribosome Binding and Sarcin/Ricin Loop Depurination by Shiga and Ricin Holotoxins.
Toxins (Basel). 2017 Apr 11;9(4):133. doi: 10.3390/toxins9040133.
8
Shiga Toxins as Multi-Functional Proteins: Induction of Host Cellular Stress Responses, Role in Pathogenesis and Therapeutic Applications.
Toxins (Basel). 2016 Mar 17;8(3):77. doi: 10.3390/toxins8030077.
9
The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1.
Infect Immun. 2015 Oct 19;84(1):149-61. doi: 10.1128/IAI.00994-15. Print 2016 Jan.
10
The non-detergent sulfobetaine-201 acts as a pharmacological chaperone to promote folding and crystallization of the type II TGF-β receptor extracellular domain.
Protein Expr Purif. 2015 Nov;115:19-25. doi: 10.1016/j.pep.2015.06.001. Epub 2015 Jun 11.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Raster3D: photorealistic molecular graphics.
Methods Enzymol. 1997;277:505-24. doi: 10.1016/s0076-6879(97)77028-9.
3
High resolution X-ray structure and potent anti-HIV activity of recombinant dianthin antiviral protein.
Arzneimittelforschung. 2004;54(10):692-702. doi: 10.1055/s-0031-1297024.
4
Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7.
J Biol Chem. 2004 Jun 25;279(26):27511-7. doi: 10.1074/jbc.M401939200. Epub 2004 Apr 9.
5
Response to Shiga toxin 1 and 2 in a baboon model of hemolytic uremic syndrome.
Pediatr Nephrol. 2003 Feb;18(2):92-6. doi: 10.1007/s00467-002-1035-7. Epub 2003 Jan 10.
6
Transport of protein toxins into cells: pathways used by ricin, cholera toxin and Shiga toxin.
FEBS Lett. 2002 Oct 2;529(1):49-53. doi: 10.1016/s0014-5793(02)03182-4.
7
Crystallisation under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9 A resolution.
Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1704-7. doi: 10.1107/s0907444902014270. Epub 2002 Sep 26.
8
Crystal structures of the complexes of trichosanthin with four substrate analogs and catalytic mechanism of RNA N-glycosidase.
Proteins. 2000 Apr 1;39(1):37-46.
9
The Protein Data Bank.
Nucleic Acids Res. 2000 Jan 1;28(1):235-42. doi: 10.1093/nar/28.1.235.
10
X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs.
Protein Sci. 1999 Sep;8(9):1765-72. doi: 10.1110/ps.8.9.1765.
Zotero 插件