新月柄杆菌中的一种特定环鸟苷 3':5'-单磷酸结合蛋白。

A specific cyclic guanosine 3':5'-monophosphate-binding protein in Caulobacter crescentus.

作者信息

Sun I Y, Shapiro L, Rosen O M

出版信息

J Biol Chem. 1975 Aug 10;250(15):6181-4.

Abstract

A binding protein specific for cyclic guanosine 3':5'-monophosphate (cyclic GMP) has been partially purified from extracts of the eubacterium Caulobacter crescentus and resolved from cyclic adenosine 3':5'-monophosphate (cyclic AMP)-binding activity. Binding of cyclic GMP is not affected by the addition of cyclic AMP or 5'-GMP, but is inhibited about 50 percent by a 50-fold molar excess of dibutyryl cyclic GMP or cyclic hypoxanthine 3':5'-monophosphate. The apparent dissociation constant for the cyclic GMP-binding protein complex is 1.1 X 10(-6) M.

摘要

一种对环鸟苷3':5'-单磷酸（环鸟苷酸）具有特异性的结合蛋白已从新月柄杆菌的提取物中部分纯化出来，并与环腺苷3':5'-单磷酸（环腺苷酸）结合活性区分开来。环鸟苷酸的结合不受环腺苷酸或5'-鸟苷酸添加的影响，但50倍摩尔过量的二丁酰环鸟苷酸或环次黄嘌呤3':5'-单磷酸会使其受到约50%的抑制。环鸟苷酸结合蛋白复合物的表观解离常数为1.1×10⁻⁶ M。