Hugo P, Boyd R L, Waanders G A, Scollay R
Department of Pathology and Immunology, Monash Medical School, Parkville.
Eur J Immunol. 1991 Nov;21(11):2655-60. doi: 10.1002/eji.1830211103.
During T cell development thymocyte subsets emerge in a defined order, reflective of their maturational stage. In this study we determined the timing of appearance of CD4+CD8+CD3high thymocytes during in vivo and in vitro embryonic development, and thymic reconstitution after cortisone treatment. In these models, CD4+CD8+CD3high cells followed CD4+CD8+CD3low and preceded mature CD4+CD8-CD3high/CD4-CD8+CD3high thymocytes, while cortisone resistance was first seen among CD4+CD8+CD3high cells. CD4+CD8+CD3high thymocytes were also shown to display a pattern of antigen receptor-mediated calcium influx intermediate between that induced in other CD4+CD8+ cells and mature thymocytes. These results are consistent with a precursor-progeny relationship between CD4+CD8+CD3low and CD4+CD8+CD3high thymocytes, the latter developing to mature thymocytes (Hugo, P. et al., Int. Immunol. 1991. 3: 265).
在T细胞发育过程中，胸腺细胞亚群按特定顺序出现，这反映了它们的成熟阶段。在本研究中，我们确定了体内和体外胚胎发育过程中以及可的松治疗后胸腺重建过程中CD4+CD8+CD3high胸腺细胞出现的时间。在这些模型中，CD4+CD8+CD3high细胞出现在CD4+CD8+CD3low细胞之后，成熟的CD4+CD8-CD3high/CD4-CD8+CD3high胸腺细胞之前，而可的松抗性首先在CD4+CD8+CD3high细胞中出现。CD4+CD8+CD3high胸腺细胞还显示出一种抗原受体介导的钙内流模式，介于其他CD4+CD8+细胞和成熟胸腺细胞所诱导的模式之间。这些结果与CD4+CD8+CD3low和CD4+CD8+CD3high胸腺细胞之间的前体-子代关系一致，后者发育为成熟胸腺细胞（雨果，P.等人，《国际免疫学》，1991年。3:265）。
