Holland P W, Williams N A, Lanfear J
Department of Zoology, University of Oxford, UK.
FEBS Lett. 1991 Oct 21;291(2):211-3. doi: 10.1016/0014-5793(91)81286-h.
We have used the polymerase chain reaction (PCR) to amplify, clone and sequence homologues of the Drosophila segment polarity genes engrailed (en), cubitus interruptus Dominant (ciD) and wingless (wg) from the genome of the brachiopod, Terebratulina retusa (Linnaeus). The deduced translation products of brachiopod en and ciD share high levels of sequence identity with their Drosophila homologues. The brachiopod wg-related clone is divergent from Drosophila wg, although clearly a member of the wg/Wnt gene family. These results indicate that structural diversity of Drosophila segment polarity genes has been evolutionarily conserved in a divergent, ancient and unsegmented animal phylum.
我们利用聚合酶链反应（PCR）从腕足动物钝圆石燕（林奈）的基因组中扩增、克隆并测序了果蝇体节极性基因engrailed（en）、显性截翅（ciD）和无翅（wg）的同源基因。腕足动物en和ciD的推导翻译产物与其果蝇同源基因具有高度的序列同一性。尽管腕足动物wg相关克隆显然是wg/Wnt基因家族的成员，但它与果蝇wg存在差异。这些结果表明，果蝇体节极性基因的结构多样性在一个不同的、古老的且无体节的动物门中在进化上得到了保守。
