Zheng Huachuan, Tsuneyama Koichi, Cheng Chunmei, Takahashi Hiroyuki, Cui Zhengguo, Murai Yoshihiro, Nomoto Kazuhiro, Takano Yasuo
Department of Pathology (1), and 221st Century COE Program, Faculty of Medicine, University of Toyama, Sugitani 2630, Toyama, Japan.
Anticancer Res. 2006 May-Jun;26(3B):2353-60.
Gastrointestinal carcinogenesis generally follows the adenoma-adenocarcinoma sequence and tumor metastasis determines the survival time of the patients. The expressions of p53 and ki-67 in gastrointestinal adenoma and adenocarcinoma (GIA) were explored and their clinicopathological significance determined.
The expressions of mutant p53 and ki-67 were examined on tissue microarray containing GIA, adjacent mucosa or adenoma and metastases by immunostaining. Their expressions were compared with the clinicopathological parameters of tumors.
The expressions of mutant p53 and ki-67 were gradually increased from gastrointestinal mucosa to adenocarcinoma through adenoma (p<0.05). Mutant p53 expression showed a positive association with depth of invasion, local invasion via vessels and lymph node metastasis of GIA (p<0.05). Ki-67 expression was positively correlated with local invasion via vessels and negatively with dedifferentiation and liver metastasis of GIA (p<0.05). The expressions of both markers in metastases of GIA were consistent with their corresponding primary foci (p < 0.05). A positive association between both markers was found in the primary foci of GIA (p<0.05).
The up-regulated expressions of mutant p53 and ki-67 are involved in the carcinogenesis and progression of GIA. They appear to be objective and effective markers to reflect the development of GIA.
胃肠道癌变通常遵循腺瘤-腺癌序列，肿瘤转移决定患者的生存时间。本研究探讨了p53和ki-67在胃肠道腺瘤和腺癌（GIA）中的表达情况，并确定其临床病理意义。
通过免疫染色检测含有GIA、邻近黏膜或腺瘤及转移灶的组织芯片上突变型p53和ki-67的表达。将其表达与肿瘤的临床病理参数进行比较。
从胃肠道黏膜到腺瘤再到腺癌，突变型p53和ki-67的表达逐渐增加（p<0.05）。突变型p53表达与GIA的浸润深度、血管局部浸润及淋巴结转移呈正相关（p<0.05）。Ki-67表达与GIA的血管局部浸润呈正相关，与GIA的去分化及肝转移呈负相关（p<0.05）。GIA转移灶中这两种标志物的表达与其相应原发灶一致（p<0.05）。在GIA原发灶中发现这两种标志物呈正相关（p<0.05）。
突变型p53和ki-67表达上调参与了GIA的发生发展过程。它们似乎是反映GIA发展的客观有效标志物。
