Tsimring L S, Volfson D, Hasty J
Institute for Nonlinear Science, University of California, San Diego, La Jolla, California 92093-0402, USA.
Chaos. 2006 Jun;16(2):026103. doi: 10.1063/1.2209571.
Transcriptional regulation in small genetic circuits exhibits large stochastic fluctuations. Recent experiments have shown that a significant fraction of these fluctuations is caused by extrinsic factors. In this paper we review several theoretical and computational approaches to modeling of small genetic circuits driven by extrinsic stochastic processes. We propose a simplified approach to this problem, which can be used in the case when extrinsic fluctuations dominate the stochastic dynamics of the circuit (as appears to be the case in eukaryots). This approach is applied to a model of a single nonregulated gene that is driven by a certain gating process that affects the rate of transcription, and to a simplified version of the galactose utilization circuit in yeast.
小基因回路中的转录调控表现出较大的随机波动。最近的实验表明，这些波动的很大一部分是由外在因素引起的。在本文中，我们回顾了几种用于对外在随机过程驱动的小基因回路进行建模的理论和计算方法。我们针对这个问题提出了一种简化方法，当外在波动主导回路的随机动力学时（真核生物中似乎就是这种情况），该方法可以使用。此方法应用于由影响转录速率的特定门控过程驱动的单个非调控基因模型，以及酵母中半乳糖利用回路的简化版本。
