Jang Mi-Hyeon, Kim Chang-Ju, Kim Ee-Hwa, Kim Myung-Gyou, Leem Kang-Hyun, Kim Jeongseon
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
J Med Food. 2006 Summer;9(2):169-74. doi: 10.1089/jmf.2006.9.169.
The roots of Platycodon grandiflorum, which belongs to the Campanulaceae family, have been used as a food material and as a traditional Oriental medicine. The effect of P. grandiflorum against lipopolysaccharide (LPS)-stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E(2 )(PGE(2)) immunoassay, nitric oxide (NO) detection assay, and interleurkin-8 (IL- 8) immunoassay on BV2 microglial cells. The aqueous extract of P. grandiflorum was shown to suppress PGE(2 )synthesis and NO production by inhibiting LPS-stimulated cyclooxygenase (COX)-2 activity and expression of inducible NO synthase (iNOS) mRNAs. In addition, the treatment with P. grandiflorum reduced the LPS-induced IL-8 release. These results suggest that P. grandiflorum inhibits PGE(2) and NO production through its suppression of LPS-induced COX-2 and iNOS expression, and also reduces IL-8 secretion by microglial cells.
桔梗属桔梗科，其根已被用作食品原料和传统的东方药物。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法、逆转录-聚合酶链反应、前列腺素E2（PGE2）免疫测定、一氧化氮（NO）检测测定以及白细胞介素-8（IL-8）免疫测定，研究了桔梗对脂多糖（LPS）刺激的炎症的影响，实验对象为BV2小胶质细胞。桔梗水提取物通过抑制LPS刺激的环氧化酶（COX）-2活性和诱导型一氧化氮合酶（iNOS）mRNA的表达，显示出可抑制PGE2的合成和NO的产生。此外，桔梗处理可减少LPS诱导的IL-8释放。这些结果表明，桔梗通过抑制LPS诱导的COX-2和iNOS表达来抑制PGE2和NO的产生，并且还可减少小胶质细胞的IL-8分泌。
