Lees Jason R, Archambault Angela S, Russell John H
Department of Molecular Biology and Pharmacology, Campus Box 8103, 660 South Euclid, Washington University, St. Louis 63110, USA.
J Neuroimmunol. 2006 Aug;177(1-2):1-10. doi: 10.1016/j.jneuroim.2006.05.024. Epub 2006 Jul 5.
T-cell invasion of the CNS is critical for the induction of a variety of autoimmune mediated neuronal diseases. We utilized blood-brain barrier (BBB) mediated exclusion of anti-CD4 antibody to define populations of encephalitogenic T-cells recovered from mouse CNS preparations as either CNS invasive or non-invasive. This separation of cells allowed flow cytometric examination of the kinetics of encephalitogenic T-cell entry past the BBB. Further experiments examined the relative contribution of EAE inflammatory conditioning of the BBB to the kinetics of T-cell adherence and migration into the CNS. Inflammatory conditioning was found to have no effect on accumulation of T-cells at the vascular interface of the BBB, but was found to increase the entry of adoptively transferred T-cells into the CNS following their initial adherence to the BBB.
T细胞侵入中枢神经系统对于引发多种自身免疫介导的神经元疾病至关重要。我们利用血脑屏障（BBB）介导的抗CD4抗体排除法，将从小鼠中枢神经系统制剂中回收的致脑炎性T细胞群体定义为中枢神经系统侵入性或非侵入性。这种细胞分离使得能够通过流式细胞术检测致脑炎性T细胞穿过血脑屏障进入的动力学。进一步的实验研究了血脑屏障的实验性自身免疫性脑脊髓炎（EAE）炎症预处理对T细胞黏附和迁移至中枢神经系统动力学的相对贡献。结果发现，炎症预处理对血脑屏障血管界面处T细胞的积聚没有影响，但在过继转移的T细胞最初黏附于血脑屏障后，发现其能增加这些T细胞进入中枢神经系统的量。
