采用高效液相色谱-紫外可见检测法测定人血浆和尿液中的加巴喷丁。
Determination of gabapentin in human plasma and urine by high-performance liquid chromatography with UV-vis detection.
作者信息
Sagirli Olcay, Cetin Sevil Müge, Onal Armağan
机构信息
Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Beyazit, 34116 Istanbul, Turkey.
出版信息
J Pharm Biomed Anal. 2006 Nov 16;42(5):618-24. doi: 10.1016/j.jpba.2006.05.012. Epub 2006 Jul 5.
A simple and reliable high-performance liquid chromatographic (HPLC) method with UV-vis detection has been developed and validated for the determination of gabapentin (GBP) in human plasma and urine. The clean up of the sample was carried out by solid-phase extraction with C18-cartridge. After the clean up procedure, the samples were pre-column derivatizated with 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS). A chromatographic separation was achieved on a C18 column with a mobile phase consisting of acetonitrile and 10mM orthophosphoric acid (pH 2.5) with isocratic elution (35:65). Baclofen was used as an internal standard (I.S.). The method developed for GBP was linear over the concentration range of 0.05-5.0 microg/ml and 0.1-10.0 microg/ml for plasma and urine, respectively. The method is precise (relative standard deviation, R.S.D. <4.05%) and accurate (relative mean error, RME <0.15%); mean absolute recoveries were 72.21% for plasma and 72.73% for urine.
已开发并验证了一种采用紫外可见检测的简单可靠的高效液相色谱（HPLC）方法，用于测定人血浆和尿液中的加巴喷丁（GBP）。样品通过C18柱进行固相萃取净化。净化程序完成后，样品用1,2-萘醌-4-磺酸钠盐（NQS）进行柱前衍生化。在C18柱上进行色谱分离，流动相由乙腈和10mM正磷酸（pH 2.5）组成，等度洗脱（35:65）。巴氯芬用作内标（I.S.）。所开发的GBP方法在血浆浓度范围为0.05 - 5.0μg/ml和尿液浓度范围为0.1 - 10.0μg/ml时呈线性。该方法精密度高（相对标准偏差，R.S.D. <4.05%）且准确度高（相对平均误差，RME <0.15%）；血浆的平均绝对回收率为72.21%，尿液的平均绝对回收率为72.73%。
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