Weber A, Harris K, Lohrke S, Forney L, Smith A L
Division of Infectious Disease, Children's Hospital and Medical Center, Seattle, Washington 98105.
Infect Immun. 1991 Dec;59(12):4724-8. doi: 10.1128/iai.59.12.4724-4728.1991.
We cloned into the structural fimbrial subunit gene from a fimbriated Haemophilus influenzae b a 1.5-kb kanamycin resistance gene. The resultant strain (RKAW5) was tested by Southern analysis, hemagglutination, and electron-micrographic examination to confirm gene inactivation. In comparison with the parent, RKAW5 exhibited a significant decrease in adherence to human buccal epithelial cells and in nasal colonization of yearling rhesus monkeys.