Anderson P W, Pichichero M E, Connor E M
Infect Immun. 1985 May;48(2):565-8. doi: 10.1128/iai.48.2.565-568.1985.
Piliated Haemophilus influenzae type b strains display an enhanced adherence to human epithelial cells in vitro. However, clinical isolates, even from mucosal sites, are seldom piliated, although piliated populations can be selected from them. Experiments with rats have led some authors to suggest that piliation does not implement colonization by H. influenzae type b. Piliated populations were obtained from 35 strains by selection for adherence to human erythrocytes. One strain, H. influenzae H305, simultaneously acquired an increased adherence to rat erythrocytes and buccal epithelial cells. In contrast to other strains, H. influenzae H305 in piliated form was more effective than in nonpiliated form in the colonization of rats by intranasal inoculation. After the piliated inoculum, however, the colonies cultured from the nasal washes were negative for erythrocyte adherence. Thus, piliated H. influenzae type b strains have an apparent advantage to initiating colonization in the rat model but may give rise to nonpiliated progeny that are more readily cultivable from the mucosal surface.
b型菌毛流感嗜血杆菌菌株在体外对人上皮细胞的黏附增强。然而，临床分离株，即使是来自黏膜部位的，很少有菌毛，尽管可以从它们中筛选出有菌毛的群体。对大鼠的实验使一些作者认为菌毛在b型流感嗜血杆菌的定植过程中不起作用。通过选择对人红细胞的黏附，从35株菌株中获得了有菌毛的群体。一株名为流感嗜血杆菌H305的菌株同时获得了对大鼠红细胞和颊上皮细胞黏附的增加。与其他菌株不同，有菌毛形式的流感嗜血杆菌H305通过鼻内接种在大鼠定植中比无菌毛形式更有效。然而，接种有菌毛的菌液后，从鼻腔冲洗液中培养的菌落对红细胞黏附呈阴性。因此，有菌毛的b型流感嗜血杆菌菌株在大鼠模型中启动定植方面具有明显优势，但可能产生更容易从黏膜表面培养的无菌毛后代。
