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包埋于湿硅胶中的人血红蛋白三级和四级构象的圆二色光谱法

Circular dichroism spectroscopy of tertiary and quaternary conformations of human hemoglobin entrapped in wet silica gels.

作者信息

Ronda Luca, Bruno Stefano, Viappiani Cristiano, Abbruzzetti Stefania, Mozzarelli Andrea, Lowe Kenneth C, Bettati Stefano

机构信息

Department of Biochemistry and Molecular Biology, University of Parma, Italy.

出版信息

Protein Sci. 2006 Aug;15(8):1961-7. doi: 10.1110/ps.062272306. Epub 2006 Jul 5.

DOI:10.1110/ps.062272306
PMID:16823042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2242581/
Abstract

The relative contributions to changes in visible and near UV circular dichroism spectra of hemoglobin of heme ligation and tertiary and quaternary conformational transitions were separated by exploiting the slowing down of structural relaxations for proteins encapsulated in wet, nanoporous silica gels. Spectral signatures, previously assumed to be characteristic of T and R quaternary states, were demonstrated to be specific to different tertiary conformations. The results support the view that ligation and allosteric effectors can modulate the structural and functional properties of hemoglobin by regulating the equilibrium between the same tertiary species within both quaternary states.

摘要

通过利用包裹在湿的纳米多孔硅胶中的蛋白质结构弛豫的减慢，分离了血红素连接以及三级和四级构象转变对血红蛋白可见和近紫外圆二色光谱变化的相对贡献。先前被认为是T态和R态四级结构特征的光谱特征，被证明是不同三级构象所特有的。这些结果支持了这样一种观点，即配体结合和变构效应剂可以通过调节两种四级结构中相同三级结构物种之间的平衡来调节血红蛋白的结构和功能特性。

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本文引用的文献

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Modulation of reactivity and conformation within the T-quaternary state of human hemoglobin: the combined use of mutagenesis and sol-gel encapsulation.人血红蛋白T-四级结构状态下反应性和构象的调节：诱变与溶胶-凝胶包封的联合应用
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Biochemistry. 2005 Jun 14;44(23):8347-59. doi: 10.1021/bi050412q.
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Crystallographic evidence for a new ensemble of ligand-induced allosteric transitions in hemoglobin: the T-to-T(high) quaternary transitions.血红蛋白中配体诱导的变构转变新组合的晶体学证据：T态到T（高）四级结构转变
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