Kopple Joel D, Wang Huiyuan, Fournier Mario, Storer Thomas, Zhang Su Mei, Song Hyun Yong, Lewis Michael
Los Angeles Biomedical Research Institute, Division of Nephrology and Hypertension, Harbor-UCLA Medical Center, Torrance, California 90501, USA.
J Ren Nutr. 2006 Jul;16(3):212-5. doi: 10.1053/j.jrn.2006.04.013.
This study was performed to assess the mRNA levels in skeletal muscle of maintenance hemodialysis (MHD) patients. Fifty-one sedentary MHD patients and 21 sedentary normal adults of similar age, gender distribution, and racial/ethnic mix as the patients were examined. The subjects had needle muscle biopsy to measure mRNA levels. They were assessed in right vastus lateralis muscle for insulinlike growth factor I (IGF-IEa and IGF-IEc), IGF-II, the IGF-I receptor, the IGF-II receptor, and myostatin. mRNA was measured by real-time polymerase chain reaction amplification of reverse transcribed cDNA. The results showed that in the MHD patients, as compared with the normal control patients, skeletal muscle mRNA levels for IGF-IEa, IGF-II, and the IGF-I receptor were significantly reduced, whereas mRNA for IGF-IEc, IGF-II receptor, and myostatin were not different than normal. We conclude that sedentary MHD patients show reductions in mRNA levels in the right vastus lateralis muscle for certain growth factor proteins, notably IGF-IEa, IGF-II, and the IGF-I receptor. These abnormalities may contribute to the sarcopenia and impaired endurance capacity, strength, and physical performance that occur in MHD patients.
本研究旨在评估维持性血液透析（MHD）患者骨骼肌中的mRNA水平。研究对51例久坐不动的MHD患者以及21例年龄、性别分布和种族/民族构成与患者相似的久坐不动的正常成年人进行了检查。受试者接受肌肉穿刺活检以测量mRNA水平。对他们右侧股外侧肌进行了胰岛素样生长因子I（IGF-IEa和IGF-IEc）、IGF-II、IGF-I受体、IGF-II受体和肌肉生长抑制素的评估。通过逆转录cDNA的实时聚合酶链反应扩增来测量mRNA。结果显示，与正常对照患者相比，MHD患者中IGF-IEa、IGF-II和IGF-I受体的骨骼肌mRNA水平显著降低，而IGF-IEc、IGF-II受体和肌肉生长抑制素的mRNA水平与正常无异。我们得出结论，久坐不动的MHD患者右侧股外侧肌中某些生长因子蛋白的mRNA水平降低，尤其是IGF-IEa、IGF-II和IGF-I受体。这些异常情况可能导致MHD患者出现肌肉减少症以及耐力、力量和身体机能受损。
