Jeong Jin-Hyeok, Park Chul-Won, Tae Kyung, Lee Seung-Hwan, Shin Dae-Hyun, Kim Kyung-Rae, Park Yong-Wook
Department of Otolaryngology, College of Medicine, Hanyang University, Seoul, Korea.
Laryngoscope. 2006 Jul;116(7):1180-4. doi: 10.1097/01.mlg.0000224345.59291.da.
The objective of this study was to investigate how the expression of the RANK-RANKL-OPG system mediates the formation and differentiation of osteoclasts and causes bone resorption in cholesteatoma.
An immunohistochemical analysis was carried out in 22 cholesteatoma tissues obtained during middle ear surgery and 15 normal postauricular skin tissues to examine the expression of RANKL and OPG.
All 22 cases of cholesteatoma and the 15 cases of normal postauricular skin expressed RANKL and OPG. The count and rate of RANKL-positive cells in cholesteatoma was significantly higher than in normal postauricular skin. The count and rate of OPG-positive cells in normal postauricular skin was significantly higher than in cholesteatoma. The ratio of the positive expression rates of RANKL and OPG in cholesteatoma was statistically higher than in normal postauricular skin.
We provide evidence suggesting that RANKL, which activates osteoclasts, plays a significant role in the mechanism of bone destruction in cholesteatoma, and that the ratio of RANKL to OPG may be a reliable indicator of bone destruction in cholesteatoma.
本研究的目的是探讨RANK-RANKL-OPG系统的表达如何介导胆脂瘤中破骨细胞的形成和分化并导致骨吸收。
对22例中耳手术中获取的胆脂瘤组织和15例正常耳后皮肤组织进行免疫组织化学分析，以检测RANKL和OPG的表达。
22例胆脂瘤病例和15例正常耳后皮肤均表达RANKL和OPG。胆脂瘤中RANKL阳性细胞的计数和比例显著高于正常耳后皮肤。正常耳后皮肤中OPG阳性细胞的计数和比例显著高于胆脂瘤。胆脂瘤中RANKL和OPG阳性表达率的比值在统计学上高于正常耳后皮肤。
我们提供的证据表明，激活破骨细胞的RANKL在胆脂瘤骨破坏机制中起重要作用，且RANKL与OPG的比值可能是胆脂瘤骨破坏的可靠指标。
