Bezrukova A G, Ostashevsky I Y
Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Apr;31(4):349-53. doi: 10.1080/09553007714550411.
The radiation yeilds of unfolding (Gconf) determined by the method of tryptophan fluorescence coincide with the radiation yields of proteolytic inactivation (Gin) for chymotrypsin-like (CT-like) enzymes on irradiation in air, both in solution and in the dry state with futher dissolution at pH7. It can be supposed that the unfolding is the main process determining the proteolytic gamma-inactivation of CT-like enzymes. It was also shown that the transition of chymotrypsin and trypsin gamma-irradiated at acid pH to neutral pH is an additional action, leading to unfolding of part of the molecules.
通过色氨酸荧光法测定的去折叠辐射产额（Gconf）与在空气中辐照时，溶液中和干燥状态下的胰凝乳蛋白酶样（CT样）酶的蛋白水解失活辐射产额（Gin）相符，且在pH7条件下进一步溶解。可以推测，去折叠是决定CT样酶蛋白水解γ失活的主要过程。研究还表明，在酸性pH下γ辐照的胰凝乳蛋白酶和胰蛋白酶向中性pH的转变是一种额外作用，导致部分分子去折叠。
