Tripp C A, Myler P J, Stuart K
Seattle Biomedical Research Institute, WA 98109-1651.
Mol Biochem Parasitol. 1991 Aug;47(2):151-6. doi: 10.1016/0166-6851(91)90174-5.
Leishmania DNA 1 (LD1) is a 27.5-kb sequence that occurs in all 91 stocks of twelve New and Old World Leishmania species examined; related sequences are present in some other kinetoplastid species. LD1 has no homology to several DNA sequences that are amplified in drug-resistant Leishmania. LD1 occurs in 3 different genomic organizations in Leishmania, depending on the stock. It is present within large (1.5-2 megabase) chromosomes in all stocks, and 74 stocks contain only this form. In 12 other stocks, LD1 also occurs in smaller (less than 550 kb) chromosomes, some of which are multicopy. Five stocks contain LD1 in multicopy circular DNA molecules in addition to the sequences found in the larger chromosome(s). Restriction fragment length polymorphisms of LD1 sequences correlate with taxonomic grouping, suggsting that LD1 is an endogenous sequence.
利什曼原虫DNA 1（LD1）是一段27.5千碱基对的序列，在所检测的新旧世界12种利什曼原虫的全部91个虫株中均有出现；在其他一些动基体目物种中也存在相关序列。LD1与在耐药利什曼原虫中扩增的几种DNA序列无同源性。根据虫株不同，LD1在利什曼原虫中有3种不同的基因组结构。在所有虫株中，它存在于大（1.5 - 2兆碱基对）染色体中，74个虫株仅含有这种形式。在另外12个虫株中，LD1也存在于较小（小于550千碱基对）的染色体中，其中一些是多拷贝的。5个虫株除了在较大染色体中发现的序列外，在多拷贝环状DNA分子中也含有LD1。LD1序列的限制性片段长度多态性与分类学分组相关，表明LD1是一种内源序列。
