体外白血病细胞分化与WT1基因表达。

In vitro leukemic cell differentiation and WT1 gene expression.

作者信息

Hernandez-Caballero E, Mayani H, Montesinos J J, Arenas D, Salamanca F, Peñaloza R

机构信息

Human Genetics Research Unit, National Medical Center, IMSS, Apdo. Postal E-014, Coahuila 5, Col Roma, CP 06703, Mexico DF, Mexico.

出版信息

Leuk Res. 2007 Mar;31(3):395-7. doi: 10.1016/j.leukres.2006.05.022. Epub 2006 Jul 7.

DOI:10.1016/j.leukres.2006.05.022

PMID:16828156

Abstract

Cell differentiation and four WT1 isoforms were assessed in CD34(+) cells from patients with acute myelogenous leukemia in presence or absence of recombinant human GM-CSF and G-CSF, on days 0, 10 and 20 of culture. We found that WT1 isoforms expression was consistently higher in AML-derived CD34+ cell-enriched cell fractions, as compared to their normal counterparts, and interestingly, in both cases, cells had differentiation towards the myeloid lineage with WT1 expression different patterns. This data suggest that WT1 expression seems to be modulated by the presence of cytokines, especially on day 20 of culture.

摘要

在培养的第0天、第10天和第20天，在有或无重组人粒细胞巨噬细胞集落刺激因子（GM-CSF）和粒细胞集落刺激因子（G-CSF）的情况下，对急性髓性白血病患者的CD34(+)细胞进行细胞分化和四种WT1亚型评估。我们发现，与正常对应物相比，AML来源的CD34+细胞富集细胞组分中WT1亚型的表达始终更高，有趣的是，在这两种情况下，细胞都向髓系谱系分化，WT1表达模式不同。这些数据表明，WT1的表达似乎受细胞因子的存在调节，尤其是在培养的第20天。