抗癌草药大黄素与DNA相互作用的电化学研究。
Electrochemical studies of the interaction of the anticancer herbal drug emodin with DNA.
作者信息
Wang Liping, Lin Lin, Ye Baoxian
机构信息
Department of Chemistry, Zhengzhou University, Zhengzhou 450052, PR China.
出版信息
J Pharm Biomed Anal. 2006 Nov 16;42(5):625-9. doi: 10.1016/j.jpba.2006.05.017. Epub 2006 Jul 7.
The interaction between anticancer herbal drug emodin and DNA was studied with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at the bare or DNA modified GCE and ultraviolet-visible (UV) spectra. The DPV of emodin showed that peak potentials shifted to more positive value and peak currents decreased with the addition of DNA. UV spectra exhibited that the absorption of emodin at about 440nm decreased with red shift. The results showed that the herbal drug emodin interacted with DNA by intercalating into the double helix of DNA. Under our experiment conditions, the decrease of peak current was proportional to DNA concentration, which can be applied to determining DNA concentration. The combining constant (beta) and combing number (m) of DNA-mEM were determined too.
采用差分脉冲伏安法（DPV）和循环伏安法（CV）在裸玻碳电极或DNA修饰的玻碳电极上以及紫外可见（UV）光谱研究了抗癌草药大黄素与DNA之间的相互作用。大黄素的DPV表明，加入DNA后，峰电位向更正的值移动，峰电流降低。UV光谱显示，大黄素在约440nm处的吸收随红移而降低。结果表明，草药大黄素通过插入DNA双螺旋与DNA相互作用。在我们的实验条件下，峰电流的降低与DNA浓度成正比，可用于测定DNA浓度。还测定了DNA-mEM的结合常数（β）和结合数（m）。
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