Piao Da-Xun, Jiang Tao, Liu Lian-Xin, Zhu An-Long, Jin Shao-Feng, Guan Ying-Hui
Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
World J Gastroenterol. 2006 Jul 14;12(26):4166-9. doi: 10.3748/wjg.v12.i26.4166.
To investigate the effect of donor splenocyte infusion combined with cyclosporine A (CsA) on rejection of rat small bowel transplantation (SBT).
Male Sprague-Dawley (SD) rats and female Wistar rats weighing 230-270 g were used as donors and recipients respectively in the study. Heterotopic small bowel transplantation was performed. The rats were divided into three groups: group one receiving allotransplantation (SD rarr Wistar), group two receiving allotransplantation (SD rarr Wistar) + donor splenocyte infusion, group three receiving allotransplantation (SD rarr Wistar) + donor splenocyte infusion + CsA followed by CsA 10 mg/kg per day after transplantation, in which recipient Wistar rats were injected with 2 x 10(8) SD splenocytes 28 d before transplantation, and treated with CsA after transplantation. Finally, the specific DNA fragment of donor Y chromosome was detected in recipient peripheral blood and skin by PCR. The survival time after small bowel transplantation was observed. Gross and histopathological examinations were performed.
The survival time after small bowel trans-plantation was 7.1 +/- 1.2 d in group 1, 18.4 +/- 3.6 d in group 2 and 31.5 +/- 3.1 d in group 3. The survival time was significant longer (P < 0.01) in group 3 than in groups 1 and 2. The gross and histopathological examination showed that the rejection degree in group 3 was lower than that in groups 1 and 2.
Donor splenocyte infusion combined with CsA decreases remarkably the rejection and prolongs the survival time after rat small bowel transplantation.
探讨供体脾细胞输注联合环孢素A（CsA）对大鼠小肠移植（SBT）排斥反应的影响。
本研究分别采用体重230 - 270 g的雄性Sprague-Dawley（SD）大鼠和雌性Wistar大鼠作为供体和受体。进行异位小肠移植。大鼠分为三组：一组接受同种异体移植（SD→Wistar），二组接受同种异体移植（SD→Wistar）+供体脾细胞输注，三组接受同种异体移植（SD→Wistar）+供体脾细胞输注+CsA，移植后每天给予CsA 10 mg/kg，其中受体Wistar大鼠在移植前28天注射2×10⁸个SD脾细胞，并在移植后用CsA治疗。最后，通过聚合酶链反应（PCR）检测受体外周血和皮肤中供体Y染色体的特异性DNA片段。观察小肠移植后的存活时间。进行大体和组织病理学检查。
一组小肠移植后的存活时间为7.1±1.2天，二组为18.4±3.6天，三组为31.5±3.1天。三组的存活时间显著长于一组和二组（P<0.01）。大体和组织病理学检查显示，三组的排斥程度低于一组和二组。
供体脾细胞输注联合CsA可显著降低大鼠小肠移植后的排斥反应并延长其存活时间。
