Herbinger Karl-Heinz, Gerhardt Martina, Piyasirisilp Sucheep, Mloka Doreen, Arroyo Miguel A, Hoffmann Oliver, Maboko Leonard, Birx Deborah L, Mmbando Donan, McCutchan Francine E, Hoelscher Michael
Department of Infectious Diseases and Tropical Medicine, Ludwig-Maximilians-University, Munich, Germany.
AIDS Res Hum Retroviruses. 2006 Jul;22(7):599-606. doi: 10.1089/aid.2006.22.599.
HIV-1 diversity, frequency of recombinants, and dual infection were determined in two populations with different HIV risk behavior. A high-risk cohort of 600 female bar workers and a normal-risk population of 1,108 antenatal clinic attendees and blood donors were recruited. Behavioral data were assessed and blood for HIV- 1 diagnosis and genotyping was sampled. HIV-1 subtypes were defined through the multiregion hybridization assay (MHA(acd)). HIV-1 prevalence differed significantly among the two populations. The prevalence was 67.8% in the population of bar workers and 17% in the normal-risk population (antenatal care attendees and blood donors). Within the normal-risk population the HIV-1 prevalence was lowest in the group of volunteer blood donors. The frequency of HIV-1 infection in women was 1.7 times higher than in men. The overall subtype distribution was A (8.5%), C (40.8%), D (3.8%), AC (25.4%), AD (5.4%), CD (8.8%), and ACD (7.3%). In the high-risk population there was a higher percentage of HIV-1 recombinant strains (54% vs. 40%, p < 0.05) and a higher frequency of dual infections (19% vs. 9%, p < 0.02) compared to the normal-risk population. High-risk populations may play an important role in the evolution of HIV, as they can provide an opportunity for the virus to coinfect, recombine, and adapt to the host-specific genetic background.
在两个具有不同HIV风险行为的人群中，对HIV-1的多样性、重组体频率和双重感染情况进行了测定。招募了600名女性酒吧工作人员组成的高风险队列以及1108名产前诊所就诊者和献血者组成的正常风险人群。评估了行为数据，并采集血液用于HIV-1诊断和基因分型。通过多区域杂交试验（MHA(acd)）确定HIV-1亚型。两个群体中的HIV-1患病率存在显著差异。酒吧工作人员群体中的患病率为67.8%，正常风险人群（产前护理就诊者和献血者）中的患病率为17%。在正常风险人群中，志愿献血者组的HIV-1患病率最低。女性中HIV-1感染频率比男性高1.7倍。总体亚型分布为A（8.5%）、C（40.8%）、D（3.8%）、AC（25.4%）、AD（5.4%）、CD（8.8%）和ACD（7.3%）。与正常风险人群相比，高风险人群中HIV-1重组毒株的比例更高（54%对40%，p<0.05），双重感染频率也更高（19%对9%，p<0.02）。高风险人群可能在HIV的进化中发挥重要作用，因为它们可以为病毒提供共同感染、重组并适应宿主特异性遗传背景的机会。
