Kato Tetsuo, Kaneko Sachiko, Kimizuka Ryuta, Okuda Katsuji
Department of Microbiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
FEMS Immunol Med Microbiol. 2006 Jul;47(2):262-6. doi: 10.1111/j.1574-695X.2006.00075.x.
The effects of exercise on serum interleukin-6 and tumor necrosis factor alpha levels were investigated using mice. Five-week-old female BALB/c mice (Th2-biased) and C57BL/6 mice (Th1-biased) were divided into exercise and control groups. The exercise group was exercised in a rotating basket type treadmill for 1 h (5 r.p.m.). Blood was collected and the serum was separated immediately after exercise. The serum interleukin-6 and tumor necrosis factor alpha levels were measured using an Endogen ELISA kit. Exercise significantly increased the serum interleukin-6 level in the two strains of mice (P<0.05 and P<0.01). The tumor necrosis factor alpha level was decreased in the exercise group. Next, periodontopathic bacterial endotoxin (lipopolysaccharide) was administered after exercise, and the effects of exercise on the lipopolysaccharide-induced serum interleukin-6 and tumor necrosis factor alpha levels were investigated. Exercise inhibited lipopolysaccharide-induced tumor necrosis factor alpha production, suggesting it has a defensive action against endotoxin shock.
利用小鼠研究了运动对血清白细胞介素 -6 和肿瘤坏死因子α水平的影响。将五周龄的雌性 BALB/c 小鼠（偏向 Th2）和 C57BL/6 小鼠（偏向 Th1）分为运动组和对照组。运动组在旋转篮式跑步机上运动 1 小时（每分钟 5 转）。运动后立即采集血液并分离血清。使用 Endogen ELISA 试剂盒测量血清白细胞介素 -6 和肿瘤坏死因子α水平。运动显著提高了两种品系小鼠的血清白细胞介素 -6 水平（P<0.05 和 P<0.01）。运动组的肿瘤坏死因子α水平降低。接下来，运动后给予牙周病细菌内毒素（脂多糖），并研究运动对脂多糖诱导的血清白细胞介素 -6 和肿瘤坏死因子α水平的影响。运动抑制了脂多糖诱导的肿瘤坏死因子α产生，表明其对内毒素休克具有防御作用。
