[In vitro preservation of laryngeal allograft for larynx transplantation].

BACKGROUND & OBJECTIVE: As a complex organ, laryngeal allograft cannot endure more than 3 hours during preservative time in vitro. This study was to explore the best preservation method and time limit of laryngeal allograft.

METHODS

During transplantation experiment of the larynx, HCA solution and UW's solution was used to perfuse laryngeal allograft on original position separately. The sequential morphologic changes of the allograft after cold preservation were observed to compare the effects of the 2 solutions.

RESULTS

After preservation in HCA solution for 20 h, ultrastructure changes, including mitochondrial edema or collapse and chromatin agglutination, appeared in mucosa, capillary and intrinsic laryngeal muscle cells of the laryngeal allograft; nuclear pyknosis was observed after 22 h and followed by apoptosis. While ischemic and necrosis changes of the laryngeal allograft appeared after preservation in UW's solution for 24 h.

CONCLUSIONS

UW's solution can preserve laryngeal allograft for 24 h in vitro, and has better effect than HCA solution.