Liu Qin-xiu, Li Qing-feng, Zheng Sheng-wu, Wang Hui-yong, Jiang Hao, Li Jiang, Zheng Dan-ning, Tang Lu-jia, Li Yu-ping, Zhang Di-sheng
Department of Plastic and Reconstructive Surgery, the Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2006 Sep;22(5):334-8.
To investigate the effective method of preserving composite facial allograft so as to attenuate ischemic injury.
The composite facial allografts were harvested from dog, perfused and preserved with 4 degrees C physiological sodium chloride and UW solution respectively. Immediately after the removal of the flap, after 12, 24, 48 h of preservation, MTT assay was used to determine the viability of several kinds of tissue, including skin, mucosa, muscle, bleed vessel, nerve and gland. The results of the two groups were compared in term of viability percentage. The pathology of several tissues were observed after 24 and 48 h of storage.
The viability percentage of every tissue conserved in UW solution for 48 hours was more than 75%. There was significant difference between physiological sodium chloride group and UW group (P < 0.05). Some changes, including Porous arrangement of fibers in connective tissue of skin and mucosa, hyalinization of tissue around the hair follicle and edema of cell in hair follicle, enlargement of space between muscle bundles and unclearness of boundary of acinus could be seen in physiological sodium chloride group while no significant change in UW group.
UW solution could be considered as preservation solution for composite facial allograft.
探讨保存异体面部复合组织移植体以减轻缺血损伤的有效方法。
从犬体获取异体面部复合组织移植体，分别用4℃生理氯化钠溶液和UW液进行灌注及保存。切取皮瓣后即刻、保存12、24、48小时后，采用MTT法检测皮肤、黏膜、肌肉、血管、神经及腺体等多种组织的活力，比较两组组织活力百分比结果。保存24、48小时后观察各组织病理学变化。
UW液保存48小时各组织活力百分比均大于75%。生理氯化钠溶液组与UW液组比较差异有统计学意义（P<0.05）。生理氯化钠溶液组可见皮肤及黏膜结缔组织纤维呈疏松排列、毛囊周围组织玻璃样变、毛囊细胞水肿，肌束间隙增宽、腺泡边界不清等改变，UW液组未见明显变化。
UW液可作为异体面部复合组织移植体的保存液。
