Demirbilek Savaş, Karaman Abdurrahman, Baykarabulut Aysun, Akin Melih, Gürünlüoglu Kubilay, Türkmen Emine, Taş Erkan, Aksoy Rauf Tugrul, Edali Mehmet Naci
Department of Pediatric Surgery, Inönü University School of Medicine, Malatya, Turkey.
Int J Urol. 2006 Jun;13(6):747-53. doi: 10.1111/j.1442-2042.2006.01397.x.
Polyenylphosphatidycholine has been demonstrated to have antioxidant, cytoprotective and anti-inflammatory effects. Whether polyenylphosphatidycholine pretreatment affects ischemia/reperfusion-induced renal damage in vivo is not known and was investigated here in rats.
Forty female Sprague-Dawley rats were divided into three groups. Group 1 (n = 10) was given saline (control, sham operated). Group 2 (n = 15) were given saline, and Group 3 (n = 15) were given polyenylphosphatidycholine (100 mg/day for 10 days prior to experiment). Groups 2 and 3 were subjected to bilateral renal ischemia (60 min) followed by reperfusion (6 h). After the reperfusion period, the rats were sacrificed and kidney tissue superoxide dismutase, glutathione, total nitrite and nitrate, malondialdehyde and myeloperoxidase levels, plasma aspartate aminotransferase, blood urea nitrogen and creatinine concentrations, and nuclear factor kappa beta expression were determined.
Serum levels of aspartate aminotransferase, blood urea nitrogen and creatinine were significantly decreased (P < 0.05) in the treatment group compared to those in the ischemic group. There were significant differences between treatment and ischemic groups regarding the tissue superoxide dismutase, glutathione, total nitrite and nitrate, malondialdehyde, and myeloperoxidase levels (P < 0.05). In addition, polyenylphosphatidycholine pretreatment reduced nuclear factor kappa beta expression in ischemic kidney tissue. Kidneys obtained from rats pretreated with polyenylphosphatidycholine demonstrated marked reduction of the histological features of renal injury compared to kidneys obtained from Group 2 rats, including a little vacuolization, pyknosis and necrosis.
Polyenylphosphatidycholine pretreatment provided significant protection against ischemia/reperfusion injury to the kidney. This treatment could be therapeutic in kidney transplantation and other conditions associated with ischemia/reperfusion injury to the kidney.
多烯磷脂酰胆碱已被证明具有抗氧化、细胞保护和抗炎作用。多烯磷脂酰胆碱预处理是否会影响体内缺血/再灌注诱导的肾损伤尚不清楚，本研究在大鼠中对此进行了探究。
40只雌性Sprague-Dawley大鼠分为三组。第1组（n = 10）给予生理盐水（对照组，假手术）。第2组（n = 15）给予生理盐水，第3组（n = 15）给予多烯磷脂酰胆碱（实验前10天每天100 mg）。第2组和第3组进行双侧肾脏缺血（60分钟），随后再灌注（6小时）。再灌注期结束后，处死大鼠，测定肾组织超氧化物歧化酶、谷胱甘肽、总亚硝酸盐和硝酸盐、丙二醛和髓过氧化物酶水平，血浆天冬氨酸转氨酶、血尿素氮和肌酐浓度，以及核因子κB表达。
与缺血组相比，治疗组血清天冬氨酸转氨酶、血尿素氮和肌酐水平显著降低（P < 0.05）。治疗组与缺血组在组织超氧化物歧化酶、谷胱甘肽、总亚硝酸盐和硝酸盐、丙二醛和髓过氧化物酶水平方面存在显著差异（P < 0.05）。此外，多烯磷脂酰胆碱预处理降低了缺血肾组织中核因子κB的表达。与第2组大鼠的肾脏相比，多烯磷脂酰胆碱预处理大鼠的肾脏显示出肾损伤组织学特征的明显减轻，包括少量空泡化、核固缩和坏死。
多烯磷脂酰胆碱预处理对肾脏缺血/再灌注损伤具有显著保护作用。这种治疗方法可能对肾移植及其他与肾脏缺血/再灌注损伤相关的疾病具有治疗作用。
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