大麻二酚系统在缺血/再灌注肾损伤动物模型中的作用评估。
Evaluation of the role of the cannabidiol system in an animal model of ischemia/reperfusion kidney injury.
作者信息
Soares Rodrigo Zon, Vuolo Francieli, Dall'Igna Dhébora Mozena, Michels Monique, Crippa José Alexandre de Souza, Hallak Jaime Eduardo Cecílio, Zuardi Antonio Waldo, Dal-Pizzol Felipe
机构信息
Laboratório de Fisiopatologia Experimental, Programa de Graduação em Ciências da Saúde, Unidade de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Departamento de Neurociências e Comportamento, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brazil.
出版信息
Rev Bras Ter Intensiva. 2015 Oct-Dec;27(4):383-9. doi: 10.5935/0103-507X.20150064.
OBJECTIVE
This work aimed to investigate the effects of the administration of cannabidiol in a kidney ischemia/reperfusion animal model.
METHODS
Kidney injury was induced by 45 minutes of renal ischemia followed by reperfusion. Cannabidiol (5mg/kg) was administered immediately after reperfusion.
RESULTS
Ischemia/reperfusion increased the IL-1 and TNF levels, and these levels were attenuated by cannabidiol treatment. Additionally, cannabidiol was able to decrease lipid and protein oxidative damage, but not the nitrite/nitrate levels. Kidney injury after ischemia/reperfusion seemed to be independent of the cannabidiol receptor 1 and cannabidiol receptor 2 (CB1 and CB2) expression levels, as there was no significant increase in these receptors after reperfusion.
CONCLUSION
The cannabidiol treatment had a protective effect against inflammation and oxidative damage in the kidney ischemia/reperfusion model. These effects seemed to be independent of CB1/CB2 receptor activation.
目的
本研究旨在探讨在肾脏缺血/再灌注动物模型中给予大麻二酚的效果。
方法
通过45分钟的肾脏缺血然后再灌注诱导肾损伤。再灌注后立即给予大麻二酚（5mg/kg）。
结果
缺血/再灌注增加了白细胞介素-1和肿瘤坏死因子水平，而这些水平通过大麻二酚治疗得到了减轻。此外，大麻二酚能够减少脂质和蛋白质的氧化损伤，但不能降低亚硝酸盐/硝酸盐水平。缺血/再灌注后的肾损伤似乎与大麻二酚受体1和大麻二酚受体2（CB1和CB2）的表达水平无关，因为再灌注后这些受体没有显著增加。
结论
在肾脏缺血/再灌注模型中，大麻二酚治疗对炎症和氧化损伤具有保护作用。这些作用似乎与CB1/CB2受体激活无关。
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