Luppi Patrizia, Tse Hubert, Lain Kristine Y, Markovic Nina, Piganelli Jon D, DeLoia Julie A
Division of Immunogenetics, Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh, School of Medicine, PA 15213, USA. luppip+@pitt.edu
Am J Reprod Immunol. 2006 Aug;56(2):135-44. doi: 10.1111/j.1600-0897.2006.00386.x.
Compelling evidence implicates peripheral immune activation in the pathophysiology of preeclampsia. Polymorphonuclear neutrophils appear to be the cells most strongly affected, with changes in expression of surface markers and release of granule enzymes. Here, we investigated activation in additional leukocyte populations among women with preeclampsia.
We used flow cytometry to evaluate changes in leukocyte markers in preeclampsia compared with uncomplicated pregnancy. To gain insights into intracellular pathways involved in leukocyte activation, we monitored the NF-kappaB signal transduction pathway. Plasma levels of interleukin-6 (IL-6) were also studied as an additional indication of cellular activation.
Preeclampsia is associated with changes in L-selectin (CD62L) on neutrophils (P = 0.004), monocytes (P = 0.013), and T cells (P = 0.048) when compared with normal pregnancy. These changes include an increase in nuclear translocation of NF-kappaB and increased levels of IL-6 (P = 0.005).
These findings are consistent with the presence of a generalized phenomenon of immune activation in preeclampsia.
有力证据表明外周免疫激活参与了先兆子痫的病理生理过程。多形核中性粒细胞似乎是受影响最严重的细胞，其表面标志物表达和颗粒酶释放发生了变化。在此，我们研究了先兆子痫女性其他白细胞群体的激活情况。
我们使用流式细胞术评估先兆子痫患者与正常妊娠女性相比白细胞标志物的变化。为深入了解白细胞激活所涉及的细胞内途径，我们监测了核因子κB信号转导途径。还研究了血浆白细胞介素-6（IL-6）水平作为细胞激活的另一指标。
与正常妊娠相比，先兆子痫与中性粒细胞（P = 0.004）、单核细胞（P = 0.013）和T细胞（P = 0.048）上L-选择素（CD62L）的变化有关。这些变化包括核因子κB核转位增加和IL-6水平升高（P = 0.005）。
这些发现与先兆子痫中存在全身性免疫激活现象一致。
