Pirzadeh P, Moosavi-Movahedi A A, Hemmateenejad B, Ahmad F, Shamsipur M, Saboury A A
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Colloids Surf B Biointerfaces. 2006 Sep 1;52(1):31-8. doi: 10.1016/j.colsurfb.2006.05.019. Epub 2006 Jun 3.
The interaction of hen egg-white lysozyme with sodium n-dodecyl sulfate (SDS) as an anionic surfactant was investigated by UV-vis spectrophotometry at different pHs at 25 degrees C using HCl/glycine and NaOH/glycine for acidic and basic pH ranges, respectively. Analysis of the spectral data using chemometric method gave the evidence for the existence of intermediate components during the cited interaction. Results also indicated a connection between turbidity of the protein solution upon interaction with SDS and distribution of our newly found intermediates. As intermediates are important in aggregation of proteins, beta-cyclodextrin was employed as an anti-aggregation agent and the results obtained for the lysozyme-SDS-beta-cyclodextrin ternary system were compared with those obtained in the absence of beta-cyclodextrin on distribution and mole fraction of intermediates with. It is also shown that as the distribution of intermediates broadens in a range of SDS concentrations, the turbidity and aggregation state of solution are reduced.
在25℃下，分别使用HCl/甘氨酸和NaOH/甘氨酸调节酸性和碱性pH范围，通过紫外可见分光光度法研究了蛋清溶菌酶与阴离子表面活性剂正十二烷基硫酸钠（SDS）的相互作用。使用化学计量学方法对光谱数据进行分析，为上述相互作用过程中存在中间组分提供了证据。结果还表明，蛋白质溶液与SDS相互作用时的浊度与新发现的中间产物分布之间存在联系。由于中间产物在蛋白质聚集过程中很重要，因此使用β-环糊精作为抗聚集剂，并将溶菌酶-SDS-β-环糊精三元体系的结果与在不存在β-环糊精的情况下获得的中间产物分布和摩尔分数结果进行比较。研究还表明，随着中间产物在一系列SDS浓度范围内的分布变宽，溶液的浊度和聚集状态会降低。
