Hagelberg E, Bell L S, Allen T, Boyde A, Jones S J, Clegg J B
MRC Molecular Haematology Unit, University of Oxford, John Radcliffe Hospital, U.K.
Philos Trans R Soc Lond B Biol Sci. 1991 Sep 30;333(1268):399-407. doi: 10.1098/rstb.1991.0090.
The analysis of DNA from ancient bone has numerous applications in archaeology and molecular evolution. Significant amounts of genetic information can be recovered from ancient bone: mitochondrial DNA sequences of 800 base pairs have been amplified from a 750-year-old human femur by using the polymerase chain reaction. DNA recovery varies considerably between bone samples and is not dependent on the age of the specimen. We present the results of a study on a small number of bones from a mediaeval and a 17th-century cemetery in Abingdon showing the relation between gross preservation, microscopic preservation and DNA recovery.
对古代骨骼DNA的分析在考古学和分子进化领域有诸多应用。从古代骨骼中可获取大量遗传信息：利用聚合酶链反应已从一根有750年历史的人类股骨中扩增出800个碱基对的线粒体DNA序列。不同骨骼样本的DNA回收率差异很大，且不取决于标本的年代。我们展示了一项对阿宾登中世纪和17世纪墓地少量骨骼的研究结果，该结果显示了大体保存、微观保存与DNA回收率之间的关系。
