Wells J, Vietje B P, McKeon R J
Department of Anatomy and Neurobiology, University of Vermont, Burlington 05405.
J Comp Neurol. 1991 Oct 8;312(2):242-50. doi: 10.1002/cne.903120207.
A naturally occurring species-specific membrane marker was used to identify unambiguously transplanted septal cells and their fibers which have grown into host tissue. Cell suspensions of the septum/basal forebrain region of C57Bl/6 mouse embryos were transplanted into the dentate gyrus of Sprague-Dawley rats that had received a fornix lesion. The membranes of the mouse contained Thy-1.2, while the membranes of the rat contained Thy-1.1. An antibody to Thy-1.2 clearly identified the donor tissue and did not react with the Thy-1.1 of the host's membranes. The ultrastructure of the immunoreactively labeled tissue confirmed previous biochemical findings on the distribution of Thy-1 and showed Thy-1.2 immunoreactivity on axons and dendrites, microtubules, some mitochondrial membranes, and the surface membranes of cell bodies. Within the transplant, a few glial profiles showed immunoreactive fibrils, but most glial profiles within the transplant and all glial profiles outside the transplant were not immunoreactive. Astrocyte fibers enclosed the outgrowing labeled fibers to form fascicles, but did not penetrate the fascicle. There was no other distinctive association of astrocytic profiles with immunoreactive fibers. Dendrites grew for long distances into the host's molecular layer. Many immunoreactive dendritic profiles formed synapses with unlabeled terminal profiles from the host. The host synapses on the long dendrites of the transplanted neurons may form an important source of input for the initiation of physiological activity in the new circuits established by the transplant. A few labeled (donor) synaptic terminals were observed in the molecular layer, but Thy-1.2-labeled dendritic profiles were much more prominent than labeled axonal profiles.
一种天然存在的物种特异性膜标记物被用于明确鉴定已移植的隔区细胞及其长入宿主组织的纤维。将C57Bl/6小鼠胚胎隔区/基底前脑区域的细胞悬液移植到接受穹窿损伤的Sprague-Dawley大鼠的齿状回中。小鼠的细胞膜含有Thy-1.2，而大鼠的细胞膜含有Thy-1.1。一种针对Thy-1.2的抗体能清晰地识别供体组织，且不与宿主细胞膜上的Thy-1.1发生反应。免疫反应标记组织的超微结构证实了先前关于Thy-1分布的生化研究结果，并显示Thy-1.2在轴突、树突、微管、一些线粒体膜以及细胞体的表面膜上具有免疫反应性。在移植物中，少数神经胶质细胞轮廓显示有免疫反应性纤维，但移植物内的大多数神经胶质细胞轮廓以及移植物外的所有神经胶质细胞轮廓均无免疫反应性。星形胶质细胞纤维围绕着长出的标记纤维形成束，但不穿透束。星形胶质细胞轮廓与免疫反应性纤维没有其他明显的关联。树突向宿主分子层中生长很长距离。许多免疫反应性树突轮廓与来自宿主的未标记终末轮廓形成突触。宿主在移植神经元长树突上形成的突触可能是移植所建立的新回路中生理活动起始的重要输入来源。在分子层中观察到少数标记（供体）的突触终末，但Thy-1.2标记的树突轮廓比标记的轴突轮廓要突出得多。
