Mercereau-Puijalon O, Jacquemot C, Sarthou J L
Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.
Acta Trop. 1991 Sep;49(4):281-92. doi: 10.1016/0001-706x(91)90079-y.
The genomic polymorphism of Plasmodium falciparum was investigated in a series of samples collected in Senegal during one transmission season. Restriction site polymorphism was studied by Southern blot analysis using six different probes. The patterns of the ribosomal RNA genes and of the gene coding for antigen 2L indicated a limited genomic polymorphism. Sequences hybridizing to the repeats of the Palo Alto/Wellcome serotype of S-antigen were found in one out of twelve isolates examined. This strain was shown to express the Palo Alto serotype. Restriction fragment length polymorphism was observed for the 332 gene and the 11.1 locus. The hybridization patterns showed that each sample had a distinct 11.1 locus. A comparison of three probes (332, 11.1 and rep20) detecting fragment length polymorphism indicated that maximum sensitivity was obtained using the subtelomeric repeats rep20; less sensitive patterns were observed using the 11.1 27 bp repeat probe. By using these three probes it was found that all samples were genetically distinct.
在一个传播季节期间于塞内加尔采集的一系列样本中，对恶性疟原虫的基因组多态性进行了研究。使用六种不同的探针通过Southern印迹分析研究了限制性位点多态性。核糖体RNA基因和编码抗原2L的基因的模式表明基因组多态性有限。在所检测的十二个分离株中，有一个分离株中发现了与S抗原的帕洛阿尔托/威康血清型重复序列杂交的序列。该菌株显示表达帕洛阿尔托血清型。观察到332基因和11.1位点的限制性片段长度多态性。杂交模式表明每个样本都有一个独特的11.1位点。对检测片段长度多态性的三种探针（332、11.1和rep20）的比较表明，使用亚端粒重复序列rep20可获得最大灵敏度；使用11.1 27 bp重复探针观察到的灵敏度较低的模式。通过使用这三种探针发现所有样本在遗传上都是不同的。
