Schagerlöf Ulrika, Wilson Greer, Hebert Hans, Al-Karadaghi Salam, Hägerhäll Cecilia
Department of Molecular Biophysics, Center for Chemistry and Chemical Engineering, Box 124, SE-221 00, Lund, Sweden.
Plant Mol Biol. 2006 Sep;62(1-2):215-21. doi: 10.1007/s11103-006-9015-0. Epub 2006 Jul 15.
Iron uptake in Arabidopsis thaliana is mediated by ferric chelate reductase FRO2, a transmembrane protein belonging to the flavocytochrome b family. There is no high resolution structural information available for any member of this family. We have determined the transmembrane topology of FRO2 experimentally using the alkaline phosphatase fusion method. The resulting topology is different from that obtained by theoretical predictions and contains 8 transmembrane helices, 4 of which build up the highly conserved core of the protein. This core is present in the entire flavocytochrome b family. The large water soluble domain of FRO2, which contains NADPH, FAD and oxidoreductase sequence motifs, was located on the inside of the membrane.
拟南芥中的铁吸收由铁螯合物还原酶FRO2介导，FRO2是一种属于黄素细胞色素b家族的跨膜蛋白。目前该家族的任何成员都没有高分辨率的结构信息。我们使用碱性磷酸酶融合方法通过实验确定了FRO2的跨膜拓扑结构。所得拓扑结构与理论预测结果不同，包含8个跨膜螺旋，其中4个构成了该蛋白的高度保守核心。这个核心存在于整个黄素细胞色素b家族中。FRO2的大的水溶性结构域位于膜内侧，该结构域包含NADPH、FAD和氧化还原酶序列基序。
