Suemori Hirofumi, Nakatsuji Norio
Laboratory of Embryonic Stem Cell Research, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Japan.
Methods Mol Biol. 2006;329:81-9. doi: 10.1385/1-59745-037-5:81.
Nonhuman primate embryonic stem (ES) cells are very important for preclinical research of the medical application of human ES cells. Because primate ES cells show significant differences from mouse ES cells, we have been optimizing protocols for the establishment and maintenance of monkey ES cells. The latest methods for derivation and culture of cynomolgus monkey ES cells are described in detail.
非人灵长类胚胎干细胞对于人类胚胎干细胞医学应用的临床前研究非常重要。由于灵长类胚胎干细胞与小鼠胚胎干细胞存在显著差异，我们一直在优化建立和维持猴胚胎干细胞的方案。本文详细描述了食蟹猴胚胎干细胞的最新分离和培养方法。
