体外从胚胎干细胞衍生肝细胞及其特性研究

Derivation and characterization of hepatocytes from embryonic stem cells in vitro.

作者信息

Ishizaka Shigeaki, Ouji Yukiteru, Yoshikawa Masahide, Nakatani Kazuki

机构信息

Program in Tissue Engineering, Department of Parasitology, Nara Medical University, Japan.

出版信息

Methods Mol Biol. 2006;330:387-99. doi: 10.1385/1-59745-036-7:387.

DOI:10.1385/1-59745-036-7:387

PMID:16846038

Abstract

From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticipated. We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term.

摘要

从肝脏治疗的角度来看，人们期望利用胚胎干细胞（ES）技术，从ES细胞中培育出大量高功能肝细胞用于细胞移植。我们探索了一种三维培养系统，在该系统中，通过转染肝细胞核因子-3β从小鼠ES细胞分化而来的肝细胞具有可长期维持的高代谢功能。