[伊红Y对子宫平滑肌肌动球蛋白复合体ATP酶活性的影响]
[Effect of eosin Y on ATPase activity of actomyosine complex of the uterus smooth muscle].
作者信息
Labyntseva R D, Kosterin S O
出版信息
Ukr Biokhim Zh (1999). 2005 Sep-Oct;77(5):57-61.
PMID:16846071
Abstract
The effect of eosin Y (2,4,5,7 - tetrabromofluorescein; 0.1-100 microM) on ATPase activity smooth muscle actomyosine was studied. The inhibition coefficient i50 of ATPase activity with eosin Y was 0.74 +/- 0.07 microM. The inhibitor decreased V(max) of actomyosine ATPase for ATP, but no influence on affinity of actomyosine for ATP was observed. It is suggested that eosin Y inhibits actomyosine ATPase activity noncompetitively in respect of ATP.
摘要
研究了曙红Y（2,4,5,7-四溴荧光素；0.1-100微摩尔）对平滑肌肌动球蛋白ATP酶活性的影响。曙红Y对ATP酶活性的抑制系数i50为0.74±0.07微摩尔。该抑制剂降低了肌动球蛋白ATP酶对ATP的V(max)，但未观察到其对肌动球蛋白与ATP亲和力的影响。提示曙红Y对ATP而言非竞争性抑制肌动球蛋白ATP酶活性。
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