Hatier R, Malaprade D, Sabolovic N, Grignon G
Laboratoire d'Histologie-Embryologie, Faculté de Médecine, Vandoeuvre-les-Nancy, France.
Arch Androl. 1991 Nov-Dec;27(3):185-95. doi: 10.3109/01485019108987669.
The histochemical and biochemical distributions of gamma-glutamyl transpeptidase (gamma-GT) were investigated in the epididymis of rats during fetal and postnatal development. In the epididymal homogenates, gamma-GT activity was detected on the fifth day after birth. A sharp increase was observed after 30 days of life in the caput homogenates. Moderate levels of the enzyme were found in the cauda epididymis. Gamma-GT is histochemically detected from the 15th day of gestation in Wolffian ducts and in 17- to 18-day-old fetuses in newly differentiated epididymal tubules. Enzyme activity, was associated with the plasma membranes (apical, lateral, and basal), was preponderant on the apical part of the epithelial cells. During the first 15 days of the postnatal life, the histochemical reaction intensities were identical from the caput to the cauda epididymidis. From the 18th day onwards, enzyme activity decreased in the corpus and in the cauda, while gamma-GT increased in the caput epididymidis, and a strong activity was found on the apical surface of epithelial cells. Weak or moderate gamma-GT activity of spermatozoa in the caput tubules, increasing steadily from caput to cauda epididymidis, suggests that gamma-GT may be related to the functional maturation of spermatozoa.
在大鼠胎儿期和出生后发育过程中，对附睾中γ-谷氨酰转肽酶（γ-GT）的组织化学和生化分布进行了研究。在附睾匀浆中，出生后第5天检测到γ-GT活性。出生30天后，附睾头匀浆中的γ-GT活性急剧增加。在附睾尾中发现该酶水平适中。从妊娠第15天起，在中肾管以及17至18日龄胎儿新分化的附睾小管中可通过组织化学方法检测到γ-GT。与质膜（顶端、侧面和基底）相关的酶活性在上皮细胞顶端部分占优势。在出生后的前15天，从附睾头到附睾尾的组织化学反应强度相同。从第18天起，附睾体和附睾尾中的酶活性下降，而附睾头中的γ-GT增加，并且在上皮细胞顶端表面发现强活性。附睾头小管中精子的γ-GT活性较弱或适中，从附睾头到附睾尾稳步增加，这表明γ-GT可能与精子的功能成熟有关。
