Shakina Yu N, Vostrikov V V, Sorokoumova G M, Selishcheva A A, Shvets V I
M. V. Lomonosov Moscow State Academy of Fine Chemical Technology.
Bull Exp Biol Med. 2005 Dec;140(6):711-3. doi: 10.1007/s10517-006-0062-y.
Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.
采用凝胶过滤法分离脂质体包裹的利福布汀和游离利福布汀。测定了利福布汀与不同磷脂组成的脂质体结合的百分比。带负电荷的磷脂的存在增加了结合程度。结合程度随离子强度的增加而降低。利福布汀与含蒽磷脂酰胆碱的脂质体孵育会伴随荧光猝灭。利福布汀的活性取决于脂质体的磷脂组成。我们的结果表明，利福布汀的结合与静电和疏水相互作用有关。
