Wolfenden Mark L, Cloninger Mary J
Department of Chemistry and Biochemistry and Center for Bioinspired Nanomaterials, Montana State University, 108 Gaines Hall, Bozeman, Montana 59717, USA.
Bioconjug Chem. 2006 Jul-Aug;17(4):958-66. doi: 10.1021/bc060107x.
Multivalent protein-carbohydrate interactions mediate a wide variety of intercellular recognition processes with high selectivity and specificity. Many synthetic multivalent molecules have been designed to mimic and to inhibit these processes. Using carbohydrate functionalized dendrimers, our goal is to devise a system where the binding activity and the degree of protein clustering induced by the glycopolymer can be readily attenuated. In this paper, dendrimers were functionalized with mixtures of mannose, glucose, and galactose. Their association with concanavalin A was studied using precipitation and hemagglutination assays. With less idealized systems where the association was not optimized, mixtures of low- and high-affinity ligands caused smaller than the theoretically determined differences in binding activity, although linear binding trends were observed. When systems were optimized so that high-affinity binding was achieved, then mixing low- and high-affinity ligands on the dendrimer's surface showed a predictable trend for lectin binding.
多价蛋白质 - 碳水化合物相互作用介导了多种具有高选择性和特异性的细胞间识别过程。许多合成多价分子已被设计用于模拟和抑制这些过程。利用碳水化合物功能化的树枝状大分子，我们的目标是设计一种系统，在该系统中，糖聚合物诱导的结合活性和蛋白质聚集程度能够容易地减弱。在本文中，树枝状大分子用甘露糖、葡萄糖和半乳糖的混合物进行功能化。使用沉淀和血凝试验研究了它们与伴刀豆球蛋白A的结合。在关联未优化的不太理想的系统中，低亲和力和高亲和力配体的混合物导致的结合活性差异小于理论确定值，尽管观察到了线性结合趋势。当系统经过优化以实现高亲和力结合时，在树枝状大分子表面混合低亲和力和高亲和力配体显示出凝集素结合的可预测趋势。
