Watkins Lucas P, Yang Haw
Department of Chemistry, University of California at Berkeley, California 94720, USA.
J Phys Chem B. 2005 Jan 13;109(1):617-28. doi: 10.1021/jp0467548.
We present a method for the analysis of optical single molecule emission data that exhibit discrete intensity jumps. This new method uses a generalized likelihood ratio test that determines the location of an intensity change point based on individual photon arrival times. This test is applied recursively to an entire single molecule intensity trajectory, thus finding each change points. Expectation-maximization clustering and the Bayesian information criterion is then used for accurate determination of the true number of states accessible to the system. This procedure allows rigorous and quantitative determination of intensity change points without the artificial time resolution limitations that arise from binning and thresholding.
我们提出了一种用于分析呈现离散强度跳跃的光学单分子发射数据的方法。这种新方法使用广义似然比检验，该检验基于单个光子到达时间来确定强度变化点的位置。此检验递归地应用于整个单分子强度轨迹，从而找到每个变化点。然后使用期望最大化聚类和贝叶斯信息准则来准确确定系统可访问的真实状态数。该过程允许严格且定量地确定强度变化点，而不会受到分箱和阈值处理所产生的人为时间分辨率限制。
